Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis

PloS One
Jasmina Marković-LipkovskiGerhard A Müller

Abstract

Rare neural cell adhesion molecule (NCAM) positive cells have been previously described within the normal human adult kidney interstitium, speculating that they could increase in the interstitium with incipient interstitial renal fibrosis (IRF). In the present study, among 93 biopsy samples of various kidney diseases, NCAM+ interstitial cells were detected in 62.4% cases. An increased number of NCAM+ cells was significantly observed only in incipient IRF compared to normal renal tissues and advanced IRF stages (p<0.001), independently of underlying diseases (p = 0.657). All three major NCAM isoforms' RT-PCR bands were visible either in normal or in kidneys with incipient IRF, albeit their mRNA expression levels measured by qRT-PCR were different. Applying qRT-PCR on pure NCAM+ cells population, obtained by laser capture microdissection, significant mRNA over-expression of NCAM140kD isoform was found in NCAM+ cells within incipient IRF (p = 0.004), while NCAM120kD and NCAM180kD isoforms were not changed significantly (p = 0.750; p = 0.704; respectively). Simultaneously, qRT-PCR also showed significant αSMA (p = 0.014) and SLUG (p = 0.004) mRNAs up-regulation within the NCAM+ cells of incipient IRF, as well as highly decreased ma...Continue Reading

References

Apr 24, 1999·Experimental Nephrology·J T Norman, L G Fine
Sep 13, 2003·The EMBO Journal·Junichi TakagiThomas Walz
Jul 28, 2005·Journal of Neurochemistry·Vladislav V KiselyovElisabeth Bock
Nov 10, 2005·Journal of Neurochemistry·Simone DiestelPatricia F Maness
Aug 5, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Frank Strutz, Gerhard A Müller
Oct 13, 2006·Journal of the American Society of Nephrology : JASN·Frank Strutz, Michael Zeisberg
Mar 6, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jasmina Marković-LipkovskiGerhard A Müller
Jul 3, 2008·Protein Science : a Publication of the Protein Society·Arthur KochoyanVladislav V Kiselyov
Sep 6, 2008·The EMBO Journal·Francois LehembreGerhard Christofori
Sep 24, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shuji MikamiYasunori Okada
Dec 30, 2009·The Journal of Cell Biology·Chiara FrancavillaUgo Cavallaro
Jul 10, 2010·Journal of Biomedicine & Biotechnology·David VansthertemGérard Toubeau
Sep 25, 2010·Journal of the American Society of Nephrology : JASN·Michael Zeisberg, Eric G Neilson
Mar 2, 2011·International Journal of Experimental Pathology·Soma Meran, Robert Steadman
Aug 3, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shuji MikamiYasunori Okada
Feb 7, 2012·Nature Medicine·Hikaru SugimotoRaghu Kalluri
Apr 27, 2012·Autoimmune Diseases·Alberto de Zubiria Salgado, Catalina Herrera-Diaz
Nov 10, 2012·PloS One·Nirmal ChhikaraSavita Yadav
Dec 21, 2012·American Journal of Physiology. Cell Physiology·Michael Zeisberg, Raghu Kalluri
Jan 29, 2013·Nature Medicine·Valerie S LeBleuRaghu Kalluri
Mar 7, 2013·The American Journal of Pathology·Mehmet Kemal TurStefan Gattenlöhner
Aug 27, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jonathan A Lindquist, Peter R Mertens
May 23, 2014·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Shasha LvGuangju Guan
Mar 31, 2015·Clinical Journal of the American Society of Nephrology : CJASN·Michael Zeisberg, Raghu Kalluri
Jun 10, 2015·Journal of the American Society of Nephrology : JASN·Tomokazu SoumaNorio Suzuki

❮ Previous
Next ❯

Citations

Oct 13, 2020·Kidney International·Tiffany N CazaChristopher P Larsen
Apr 4, 2021·Biomolecules·Yan GuDamu Tang

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
PCR
biopsies
reverse transcription PCR
laser capture microdissection

Software Mentioned

AxioVision
SPSS
PrimerDesign
AnalySIS

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Related Papers

International Journal of Clinical and Experimental Pathology
Sanja ĆirovićJasmina Marković-Lipkovski
Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Jasmina Marković-LipkovskiGerhard A Müller
© 2021 Meta ULC. All rights reserved