Variable gene family usage of protective and non-protective anti-Vibrio cholerae O1 LPS antibody heavy chains

Microbiology and Immunology
Terri K Wade, William F Wade

Abstract

Vibrio cholerae causes cholera, an enteric disease of humans that is a worldwide problem. The O1 serogroup of Vibrio cholerae contains two predominant serotypes (Inaba and Ogawa) of LPS, a proven protective antigen for humans and experimental animals. We generated B-cell hybridomas from mice immunized with either: (i) two doses of purified Inaba LPS; (ii) two doses of an Inaba hexasaccharide conjugate (terminal six perosamine bound to a protein carrier), (iii) four doses of purified Inaba LPS; or (iv) a low dose of purified Inaba LPS followed by a booster with the Inaba conjugate. We showed previously that the first and third immunization protocols induce vibriocidal antibodies, as does the fourth; the second protocol induces antibodies that bind Inaba and Ogawa LPS but are not vibriocidal. Anti-LPS mAbs derived from hybridomas resulting from each immunization protocol were characterized for binding to Inaba and Ogawa LPS, their vibriocidal or protective capacity, and the variable heavy chain family they expressed. LPS immunogens selected different LPS-specific B cells expressing six different Vh chain families. Protective and non-protective mAbs could express variable regions from the same family. One mAb was specific for Inab...Continue Reading

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Oct 3, 2006·FEMS Immunology and Medical Microbiology·Terri K WadeWilliam F Wade
Nov 2, 2006·American Journal of Reproductive Immunology : AJRI·William F Wade

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Citations

Dec 2, 2011·Annals of the New York Academy of Sciences·Andrea CeruttiKang Chen
Apr 1, 2014·Cell·Yasmine Belkaid, Timothy W Hand
May 23, 2012·FEMS Immunology and Medical Microbiology·Meghan MuseWilliam F Wade
Aug 24, 2013·Pathogens and Disease·William F WadeLouis B Justement
Apr 20, 2017·Immunity·Yasmine Belkaid, Oliver J Harrison

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