Variable number of tandem repeats of the insulin gene determines susceptibility to latent autoimmune diabetes in adults

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
Gloria Edith CerroneGustavo Daniel Frechtel

Abstract

The different clinical presentations of latent autoimmune diabetes in adults (LADA) and type 1 diabetes mellitus may be the result of susceptibility genes in determining the mode of onset. We analyzed the 5' polymorphisms of the insulin mini-satellite region (INS), a variable number of tandem repeats (VNTR) [repeat units; RU]. We evaluated the association of the different INS-VNTR alleles in patient susceptibility to LADA autoimmune diabetes. To our knowledge, this constitutes the first study of this kind performed in a Caucasian population. From an group of 160 Argentinean patients previously characterized as having LADA, we selected 44 patients who presented with humoral autoimmunity for genotyping and compared them to 88 patients with type 1 diabetes and 138 healthy individuals. The INS-VNTR allele classes were determined by Southern blotting (class I: 21-44RU; class III: 138-159RU). Subjects with class I alleles were further studied using PCR amplification to determine the exact length of the alleles (short 1S: 22-37RU; medium 1M: 38-41RU; large 1L: 42-43RU). Allelic and genotype frequencies were estimated by chi(2) tests for independence with 2 x 2 contingency tables and the relative risks (RR) were determined using GraphP...Continue Reading

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Citations

Sep 30, 2005·Diabetologia·S FourlanosL C Harrison
Jun 15, 2007·Immunological Investigations·Mariela CaputoDaniel Frechtel Gustavo
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Ramachandra G NaikJerry P Palmer
Jul 6, 2007·The Journal of Heredity·Andrea D ShortWilliam E R Ollier

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