Variable-number tandem repeats typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 in Thailand

Tuberculosis
Arunee Thong-OnPrasit Palittapongarnpim

Abstract

Spoligotyping and variable-number tandem repeats (VNTR) typing have been increasingly used for differentiating Mycobacterium tuberculosis strains with low copy numbers of IS6110. However, there are few studies comparing their potential to type the strains originating from South and Southeast Asia where many of the isolates have only a few copies, or even single copy, of IS6110. Here, we evaluated the genotyping of 187M. tuberculosis isolates harboring 1-6 copies of IS6110, available from a population-based study in Chiangrai, northern Thailand during 1998-2000, using spoligotyping and VNTR typing. The low-copy-number isolates constituted about 34% of all M. tuberculosis isolated in the province. Discriminating capacities and cluster identification by the two methods were compared with each other and to those obtained by the standard IS6110-restriction fragment length polymorphism (RFLP) method. We found that VNTR typing based on the studied 10-loci set generated more distinct patterns (151 patterns) than spoligotyping (54 patterns) and IS6110-RFLP (65 patterns). Most of the RFLP- or spoligotyping-defined clusters were subdivided by VNTR typing. Combining IS6110-RFLP with VNTR typing produced 164 distinct patterns and 21.9% of c...Continue Reading

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Citations

May 17, 2014·Research in Veterinary Science·E GormleyS Rodriguez-Campos
Jul 21, 2016·Journal of Global Antimicrobial Resistance·Nat SmittipatPrasit Palittapongarnpim
Jan 24, 2014·BioMed Research International·Anna ŻaczekAnna Sajduda
Apr 11, 2014·BioMed Research International·Ruqaya Mustafa AliDaniela M Cirillo
Sep 8, 2017·Journal of Human Genetics·Yosuke OmaeSurakameth Mahasirimongkol
Dec 19, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Cláudio José AugustoSilvana Spíndola de Miranda

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