PMID: 39907Aug 1, 1979

Variable pH dependence of the myosin-ATPase in different muscles of the rat

Histochemistry
M Müntener

Abstract

For the histochemical demosntration of the Myosin-ATPase (EC 3.6.1.3) the pH of both the preincubation and the incubation medium was varied in steps of 1 within a range: 10.2 to 10.5 and 9.3 to 9.9, respectively. The optimum combinations of both pH values, defined as the ones providing most consistent contrast among the three major types of muscle fibers were determined in 9 different muscles of the rat. The spectrum of optimum combinations differs considerably from muscle to muscle. The reduction of the incubation pH by only 0.1 may drastically change the staining pattern. This probably reflects the unspecificity of the histochemical procedure as well as the plasticity of the ATPase systems. To cope with the lability of the myosin-ATPase the optimum pH values of both media should be determined for each muscle separately.

References

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Citations

Jan 1, 1984·Histochemistry·A J LuginbuhlRobert S Staron
Aug 1, 1987·Pflügers Archiv : European journal of physiology·H BaumannM C Schaub
Nov 1, 1987·Pflügers Archiv : European journal of physiology·S Egginton
Oct 1, 1994·Neuropathology and Applied Neurobiology·M Olive, I Ferrer
Dec 1, 1994·Anatomia, histologia, embryologia·J L RiveroE Agüera
Jan 1, 1990·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MüntenerH Walt
Feb 1, 1985·Muscle & Nerve·M MüntenerC W Heizmann
Nov 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B R WeberM Müntener
Apr 1, 1995·Microscopy Research and Technique·N Hämäläinen, D Pette
Sep 1, 1982·Experimental Neurology·M Müntener
Jan 1, 1984·Archives of Oral Biology·J T RokxH W Jansen
Apr 15, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Flurin ItemMarco Toigo
Mar 1, 2003·Histochemistry and Cell Biology·Seth S JumpRobert S Staron
May 1, 1988·The American Journal of Physiology·D SmithM Sharratt

Related Concepts

DNA-dependent ATPase
Metazoa
Drug Stability
Cytochemistry
Hydrogen-Ion Concentration
Muscle
Actomyosin Adenosinetriphosphatase
Tissue Specificity
Rats, Laboratory

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