Variable region light chain genes encoding human antibodies to HIV-1

Molecular Immunology
D David, M Zouali


In previous work, it was found that the heavy chain variable gene (VH) repertoire of human antibodies to HIV is markedly skewed and that the gp120 molecule is a ligand for VH3 gene products. Here, we have analysed the light chain (L-chain) variable region genes (VL) expressed by a panel of human monoclonal antibodies derived from an immunized volunteer, an AIDS patient and seropositive asymptomatic donors, and specific for HIV-1 p25, gp41 and gp120 proteins. We found that, in contrast to VH gene-family use, the VL repertoire does not exhibit a family-bias. We noticed however, a tendency to the use of VL genes that map to the downstream portion of the kappa locus. The VL genes expressed have mutated at lower rates than the corresponding VH genes and show no clustering of the replacement mutations in the hypervariable regions. We also found that the third hypervariable regions (CDR3) of the L-chains have undergone a marked diversification, with addition of untemplated nucleotides, frequent truncation at the 3' end of the VLs and somatic mutation. These molecular events result in a length heterogeneity of the CDR3s and an apparently positive selection of specific highly reactive amino acids. We conclude that the specificity of, at...Continue Reading


Oct 1, 1992·The Journal of Clinical Investigation·W A MarascoJ Sodroski
Apr 1, 1992·European Journal of Immunology·A Lautner-RieskeH G Zachau
Sep 1, 1990·Molecular Immunology·V GuigouF Fumoux
Feb 1, 1992·The Journal of Clinical Investigation·A ShiraiD M Klinman
Aug 1, 1990·The Journal of Experimental Medicine·T J Vasicek, P Leder
Feb 1, 1986·The Journal of Experimental Medicine·H ChangG F Hollis
Dec 1, 1986·The Journal of Experimental Medicine·V RadouxD A Carson
Oct 1, 1988·Immunological Reviews·C Berek, C Milstein
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P P ChenD A Carson
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DariavachM P Lefranc
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A KarpasD Gilgen
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M J ShlomchikM G Weigert
Jan 1, 1987·AIDS Research and Human Retroviruses·I WendlerG Hunsmann
Dec 21, 1984·Nucleic Acids Research·M Pech, H G Zachau
Oct 1, 1993·Scandinavian Journal of Immunology·S MüllerH Köhler
Dec 1, 1993·European Journal of Immunology·R KleinH G Zachau
Oct 6, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Suzanne ShustermanPaul M Sondel

❮ Previous
Next ❯


May 1, 1996·Molecular Immunology·M Ohlin, C A Borrebaeck
Sep 29, 1999·Immunology Today·F J Stevens, Y Argon
Sep 9, 2015·The Journal of Infectious Diseases·Mohammad M SajadiGeorge K Lewis
Feb 3, 2007·Immunobiology·Mabel RodríguezAna María Vázquez
Oct 6, 2000·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·F J Stevens
Apr 24, 1999·Applied Biochemistry and Biotechnology·L JuompanM Zouali
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·David H MargolinNorman L Letvin

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.