Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections

Kinetoplastid Biology and Disease
F ClaesPhilip Büscher

Abstract

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). RESULTS: This PCR yielded a 205 bp product in all T. evansi and in seven out of nine T. equiperdum strains tested. This product was not detected in the DNA from T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. congolense, T. vivax and T. theileri parasites. The Rotat 1.2 PCR detects as few as 10 trypanosomes per reaction with purified DNA from blood samples, i.e. 50 trypanosomes/ml. CONCLUSION: PCR amplification of the RoTat 1.2 VSG gene is a specific marker for T. evansi strains, except T. evansi type B, and is especially useful in dyskinetoplastic strains where kDNA based markers may fail to amplify. Furthermore, our data support previous suggestions that some T. evansi stocks have been previously misclassified as T. equiperdum.

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Citations

Sep 20, 2011·Parasitology Research·Nguyen Thu ThuyNoboru Inoue
Jun 8, 2010·Tropical Animal Health and Production·Fikru Regassa GariFilip Claes
Feb 26, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nguyen Van Vinh ChauStephen Baker
Aug 5, 2011·The American Journal of Tropical Medicine and Hygiene·Archana VermaJacob Puliyel
Apr 5, 2005·Experimental Parasitology·J M NgairaJ J N Ngeranwa
Jun 26, 2007·Parasitology Research·Z K Njiru, C C Constantine
Jan 24, 2020·The Journal of Veterinary Medical Science·Afraa ElataKeisuke Suganuma
May 17, 2019·Parasites & Vectors·Philippe BüscherNick Van Reet
Apr 2, 2016·PLoS Neglected Tropical Diseases·Hadush BirhanuNick Van Reet
Apr 26, 2017·Tropical Animal Health and Production·Yonas GizawTeka Fayera
May 26, 2017·Genome Biology and Evolution·Bart CuypersStijn Deborggraeve
Jan 11, 2021·Veterinary Parasitology·Nathália Anderson CalomenoLuiz Claudio Miletti
May 1, 2021·Acta Tropica·Viet-Linh NguyenDomenico Otranto
Jul 6, 2021·Frontiers in Veterinary Science·Keneth Iceland KasoziSusan Christina Welburn
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·A G S ChanduP Roy
Jul 21, 2021·Experimental Parasitology·Limo William KipkorirAdung'a Vincent Owino
Sep 15, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Z K NjiruW C Gibson
Aug 17, 2021·Microbial Genomics·Guy OldrieveKeith R Matthews

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Methods Mentioned

BETA
PCR
PCRs
Genotyping
electrophoresis

Software Mentioned

AnTat
Imagemaster Video Detection System

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