Variance in expression and localization of sex-related genes CgDsx, CgBHMG1 and CgFoxl2 during diploid and triploid Pacific oyster Crassostrea gigas gonad differentiation.

Gene
Chenyang YueHong Yu

Abstract

Several evolutionarily conserved classes of transcriptional regulators were involved in diverse sex determination and differentiation pathways across taxa, whereas their roles in most mollusks is still limited. The Pacific oyster Crassostrea gigas, a dioecious bivalve with sex reversal, could be an ideal model for this issue because of its complex sexuality and potential disruption of sex differentiation in triploid individuals. Here, two mRNA splicing isoforms of a DM domain gene CgDsx and two isoforms of a novel sex-related CgBHMG1 (ortholog of BHMG1 in mammals) were identified in C. gigas. Real time PCR showed that two isoforms of CgDsx and one isoform of CgBHMG1 displayed male-specific expression in diploid oysters, opposite with the female-specific CgFoxl2 (a potential factor of female gonadic differentiation). Interestingly, the four sex-specific transcripts in diploid oyster were expressed in triploid oysters with opposite sex, triploid hermaphrodites and individuals at stage I that sex could not be determined. Subsequent in situ hybridization analysis on gonads of diploid oysters revealed predominant expression of CgDsx in spermatogonia of testes, CgBHMG1 in spermatocytes of testes and follicle cells of ovaries, and CgF...Continue Reading

References

Jan 1, 1993·Developmental Biology·V A Jursnich, K C Burtis
Dec 10, 1999·American Journal of Medical Genetics·B HuangJ Bartley
Apr 3, 2001·American Journal of Human Genetics·B K JordanE Vilain
Apr 2, 2004·Nature·Jane GrimwoodSusan M Lucas
Nov 2, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marina S GovorounCorinne Cotinot
Sep 30, 2005·Development Genes and Evolution·Craig R MagieMark Q Martindale
Apr 25, 2006·Biochemical and Biophysical Research Communications·Masatoshi NakamotoNaoki Shibata
May 2, 2006·Molecular Genetics and Metabolism·Nina Henriette Uhlenhaut, Mathias Treier
Nov 4, 2006·Developmental Biology·Qiang TuPaola Oliveri
Jan 9, 2008·American Journal of Human Genetics·Hannah MandelEli Sprecher
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 16, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amine NaimiPascal Sourdaine
May 27, 2009·PLoS Biology·Deanna M ChurchUNKNOWN Mouse Genome Sequencing Consortium
Jun 2, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Amine NaimiPascal Sourdaine
Jul 22, 2011·Nature·Clinton K MatsonDavid Zarkower
Sep 17, 2011·Current Biology : CB·Amaury Herpin, Manfred Schartl
Oct 29, 2011·Biology of Reproduction·Guan-Chung WuChing-Fong Chang
Mar 20, 2012·Trends in Genetics : TIG·Artyom Kopp
Apr 28, 2012·Current Biology : CB·Tony Gamble, David Zarkower
May 11, 2012·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·C SanterreA-S Martinez
Jan 22, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Francesc Piferrer
Oct 15, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christelle SanterreAnne-Sophie Martinez
Apr 8, 2015·Bioscience, Biotechnology, and Biochemistry·Jianwu ShiJunhua Wang
Nov 6, 2015·Physiological Reviews·Michael D Griswold
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jan 17, 2017·Asian Journal of Andrology·Shengsong HuangHaolin Chen
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein
Feb 8, 2020·Developmental Cell·Kei-Ichiro IshiguroHitoshi Niwa
Feb 26, 2020·Developmental Cell·Jon M Oatley, Michael D Griswold
Nov 13, 2020·Physiological Reviews·Yoshitaka NagahamaMasaru Nakamura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.