Variance-mean analysis in the presence of a rapid antagonist indicates vesicle depletion underlies depression at the climbing fiber synapse

Neuron
Kelly A Foster, Wade G Regehr

Abstract

Many types of synapses throughout the nervous system are transiently depressed during high-frequency stimulation. Several mechanisms have been proposed to account for this depression, including depletion of release-ready vesicles. However, numerous studies have raised doubts about the importance of depletion in depression of central synapses and have implicated alternative mechanisms, such as decreased release probability. We use variance-mean analysis to determine the mechanism of depression at the climbing fiber to Purkinje cell synapse. We find that postsynaptic receptor saturation makes it difficult to distinguish between a decrease in available vesicles and a reduction in release probability. When AMPA receptor saturation is relieved with a low-affinity antagonist, variance-mean analysis reveals that depression arises from a decrease in the number of release-ready vesicles. Vesicle depletion is prominent, despite numerous docked vesicles at each release site, due to multivesicular release. We conclude that vesicle depletion can contribute significantly to depression of central synapses.

References

Jan 1, 1989·Annual Review of Neuroscience·R S Zucker
Mar 1, 1996·Trends in Neurosciences·M V Jones, G L Westbrook
Feb 17, 2000·Trends in Neurosciences·J D Clements, R A Silver
Oct 31, 2001·Neuron·J I Wadiche, C E Jahr
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y HumeauB Poulain
Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
May 10, 2002·Trends in Neurosciences·Ralf SchneggenburgerErwin Neher
Jun 11, 2002·Nature Neuroscience·Thomas G OertnerKarel Svoboda
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rossella Conti, John Lisman
Jan 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gong ChenRichard W Tsien

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Citations

Apr 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael J McConnellCarla J Shatz
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fumiko Kawasaki, Richard W Ordway
Sep 16, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Michael C Andresen, James H Peters
Jan 29, 2010·Journal of Neurophysiology·Soham Chanda, Matthew A Xu-Friedman
Mar 28, 2008·Journal of Neurophysiology·Hua Yang, Matthew A Xu-Friedman
Jun 20, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jun DingD James Surmeier
Apr 26, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeremy F AthertonMark D Bevan
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cecilia G PhillipsStephen M Smith
May 28, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Martin PaukertDwight E Bergles
Oct 11, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ben Nahir, Craig E Jahr
Mar 30, 2011·Neural Networks : the Official Journal of the International Neural Network Society·Sergiy KochubeyLeonid Savtchenko
Mar 15, 2016·Journal of Neuroscience Methods·Monica S Thanawala, Wade G Regehr
Nov 27, 2008·The European Journal of Neuroscience·Gen Ohtsuki, Tomoo Hirano
Dec 27, 2005·Journal of Neuroscience Methods·Chiara Saviane, R Angus Silver
Jun 24, 2015·Trends in Neurosciences·Stephanie RudolphJacques I Wadiche
Jun 11, 2011·Neuron·Stephanie RudolphJacques I Wadiche
Dec 14, 2011·Neuron·Katarzyna MiśkiewiczPatrik Verstreken
Aug 1, 2019·ELife·Jada H VadenJacques I Wadiche
Dec 10, 2016·Frontiers in Computational Neuroscience·Alex D BirdMagnus J E Richardson
Oct 14, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiao-Lei ZhangTomás R Guilarte
Apr 9, 2020·Frontiers in Cellular Neuroscience·Kohgaku EguchiRyuichi Shigemoto
May 24, 2019·Frontiers in Cellular Neuroscience·Christina PätzJens Eilers

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