Variant Amino Acid Residues Alter the Enzyme Activity of Peanut Type 2 Diacylglycerol Acyltransferases

Frontiers in Plant Science
Ling ZhengZhenying Peng

Abstract

Diacylglycerol acyltransferase (DGAT) catalyzes the final step in triacylglycerol (TAG) biosynthesis via the acyl-CoA-dependent acylation of diacylglycerol. This reaction is a major control point in the Kennedy pathway for biosynthesis of TAG, which is the most important form of stored metabolic energy in most oil-producing plants. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the peanut cultivar 'Luhua 14.' Sequence analysis of 11 different peanut cultivars revealed a gene family of 8 peanut DGAT2 genes (designated AhDGAT2a-h). Sequence alignments revealed 21 nucleotide differences between the eight ORFs, but only six differences result in changes to the predicted amino acid (AA) sequences. A representative full-length cDNA clone (AhDGAT2a) was characterized in detail. The biochemical effects of altering the AhDGAT2a sequence to include single variable AA residues were tested by mutagenesis and functional complementation assays in transgenic yeast systems. All six mutant variants retained enzyme activity and produced lipid droplets in vivo. The N6D and A26P mutants also displayed increased enzyme activity and/or total cellular fatty acid (FA) content. N6D mutant mainly increased the content of pa...Continue Reading

References

May 1, 1992·Plant Molecular Biology·A R Slabas, T Fawcett
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·S CasesR V Farese
Dec 22, 1999·European Journal of Biochemistry·P Bouvier-NavéH Schaller
Dec 14, 2001·The Journal of Biological Chemistry·Line SandagerSten Stymne
Jan 25, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hui-Wen WangShou-Yi Chen
Feb 3, 2007·Lipids·Shiu-Cheung Lung, Randall J Weselake
Jul 12, 2008·Phytochemical Analysis : PCA·Giorgio GambinoIvana Gribaudo
Jul 18, 2008·Plant Physiology·Kathryn LardizabalKristen Bennett
Jul 25, 2009·Biotechnology Advances·Randall J WeselakeJohn L Harwood
Jan 19, 2010·Plant Biotechnology Journal·Runzhi LiDavid F Hildebrand
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Timothy P DurrettMike Pollard
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY916129
AAX62735.1
CAB44774.1
AAY40784.1
AAV31083.1
ADF57328.1
BAE93461.1
ABU50328.1
AAW51456.1
ABN09107.1

Methods Mentioned

BETA
transgenic
PCR
Fluorescence
Assay

Software Mentioned

Image
DNAMAN
ChromPerfect
Pro plus
TMHMM
BLAST
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.