Variant CD44 expression by human fibroblasts

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Abirami RajarajanEdward W Odell

Abstract

CD44 is a cell surface glycoprotein with roles in tumour invasion and metastasis. CD44 is variably spliced from ten variant exons and mis-splicing is a biomarker for detection of colon, urothelial and other carcinomas. Fibroblasts are normally considered to lack variant exons and thus should not generate false-positive signals. Transcription of variant exons by fibroblasts was investigated by exon-specific reverse transcription-polymerase chain reaction (RT-PCR) for variant exons v2-v10 using normal primary fibroblasts, immortalized and experimentally transformed fibroblasts. Flow cytometry, immunocytochemistry and Western blotting were used to determine expression. All types of fibroblasts, including normal primary culture fibroblasts, transcribed low levels of variant exon mRNA. Expression could not be detected by blotting or immunocytochemistry but flow cytometry revealed minor expression of some exons by all three types of cultured fibroblast. Fibroblasts do transcribe and express small amounts of variant exon CD44. This may need to be considered when using exon splicing as a biomarker for malignancy in clinical samples containing connective tissue.

References

Nov 1, 1991·Molecular and Cellular Biology·E Shtivelman, J M Bishop
Dec 1, 1989·Immunology Today·B F HaynesS M Denning
Apr 29, 1994·Biochemical and Biophysical Research Communications·L FinnD L Cooper
Jan 1, 1993·Journal of Cellular Biochemistry. Supplement·D Tarin, Y Matsumura
Jul 1, 1994·Cell Adhesion and Communication·A W GriffioenS T Pals
May 16, 1996·International Journal of Cancer. Journal International Du Cancer·D L HudsonF M Watt
Jan 1, 1997·Advances in Cancer Research·D NaorD Ish-Shalom
Jul 1, 1997·European Journal of Immunology·D R CroftI M Kramer
Dec 6, 1997·International Journal of Cancer. Journal International Du Cancer·V PatelW A Yeudall
Apr 2, 1998·Histopathology·S Goodison, D Tarin
Oct 20, 1998·The American Journal of Pathology·S GoodisonD Tarin
Jun 17, 1999·International Journal of Cancer. Journal International Du Cancer·J A FoekensS C Henzen-Logmans
Jul 20, 1999·Experimental and Molecular Pathology·A Herrera-Gayol, S Jothy
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·M MersmannD Moosmayer
Mar 2, 2002·The Journal of Laboratory and Clinical Medicine·Hong-Min NiShing Chuan Hooi
Dec 21, 2002·Differentiation; Research in Biological Diversity·Margareta M Mueller, Norbert E Fusenig
Jan 18, 2007·Journal of Cellular Biochemistry·Hanchen LiJeanmarie Houghton

❮ Previous
Next ❯

Citations

Nov 3, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Ching-Yu YenHsueh-Wei Chang
Nov 26, 2009·Ultrastructural Pathology·Gianandrea PasquinelliMichele Bisceglia
Aug 17, 2020·Biomolecules·Svenja Kleiser, Alexander Nyström
Oct 6, 2017·Molecular Pharmaceutics·Kush N ShahYang H Yun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.