Variants of gamma-glutamyl transferase in human urine

Clinica Chimica Acta; International Journal of Clinical Chemistry
K RambabuT N Pattabiraman

Abstract

When dialyzed human urine was subjected to ultracentrifugation, gamma glutamyl transferase activity amounting to one-third or less of the total activity was found associated with the microsomal fraction and the remaining in the 100 000 X g supernatant. By ion-exchange chromatography on DEAE-cellulose at pH 7.5, the urinary enzyme was resolved into two fractions. The less anionic form (eluted with 0.15 mol/l NaCl) corresponded to the 100 000 X g supernatant fraction. The more anionic form (eluted with 0.5 mol/l NaCl) corresponded to the microsomal fraction. On chromatography on concanavalin A-sepharose, the urinary transferase activity separated into the unbound fraction and the bound fraction (eluted with alpha-methyl-D-glucoside). The bound fraction was retained more tightly on DEAE-cellulose (eluted with 0.5 mol/l NaCl). The unbound fraction had weaker interaction with the ionic-exchanger (eluted with 0.15 mol/l NaCl). Each of two fractions separated by affinity chromatography on concanavalin A-sepharose, resolved into two bands on cellulose acetate electrophoresis at pH 7.t. In addition, during gel chromatography on Sephadex G-200, they separated into a high molecular weight (greater than 200 000) fraction and a low molecula...Continue Reading

References

Dec 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Rambabu, T N Pattabiraman
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S S Tate, A Meister
Feb 28, 1978·Biochemical and Biophysical Research Communications·S HoriuchiY Morino
Jul 1, 1978·Journal of Clinical Pathology·P R WenhamH G Sammons
Nov 23, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Patel, P O'Gorman
Apr 15, 1972·Klinische Wochenschrift·A Lévy, U C Dubach
Nov 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Szewczuk

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Citations

Nov 24, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·L Salgó, A Szabó
Apr 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Jung, G Schulze
Nov 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·H MatsukuraN Sakuragawa
Aug 1, 1988·Biochemical Medicine and Metabolic Biology·K RambabuM M Ziu
Jan 5, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Linder, P Sudaka

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