Variation in daily shedding patterns of Staphylococcus aureus in naturally occurring intramammary infections

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Jennifer B WalkerFred J DeGraves

Abstract

The goal of the current prospective field study was to examine the shedding patterns of naturally occurring Staphylococcus aureus intramammary infections and the association of pulsed field gel electrophoresis pulsotype with shedding. Milk samples from 5 multiparous and 2 primiparous cows identified with S. aureus intramammary infections were collected for 21 consecutive days, 3 times throughout the lactation (63 days total). Cyclicity of each quarter was evaluated using a locally weighted regression. Pulsed field gel electrophoresis was used for genotypic cluster comparisons to evaluate the association of strain type and shedding patterns. Although the amount of shedding varied greatly, 97.5% of the samples were culture positive. There were notable differences in S. aureus shedding patterns among cows as well as within cows; however, no consistent cyclic pattern was identified. Quarters infected with S. aureus isolates grouped in genotypic cluster 1 appeared to shed at consistently higher levels with a median cfu/0.01 ml of 154 (ln[cfu] = 5.0). In comparing ln(cfu)/0.01 ml between genotypic clusters over the first 21-day sample period, accounting for the effect of sample day, samples collected from quarters infected with S. au...Continue Reading

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Sep 3, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jennifer B WalkerFred J DeGraves

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Citations

May 3, 2013·Journal of the American Veterinary Medical Association·Jennifer B WalkerPäivi J Rajala-Schultz
Jun 21, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M H Fabres-KleinA O B Ribon
Jun 7, 2016·Journal of Dairy Science·M F AddisS Uzzau
Aug 16, 2016·Journal of Dairy Science·M F AddisS Uzzau
Jul 20, 2021·Journal of Dairy Science·Z DengG Koop

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Methods Mentioned

BETA
genotyping
electrophoresis

Software Mentioned

BioNumerics

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