PMID: 8372810Aug 1, 1993

Variation in DNA content of immature normal bone marrow cells

American Journal of Hematology
S WidellP Reizenstein


A variability in DNA content detected was found with image cytometry, in immature bone marrow cells from 13 healthy donors (median age 31 yr). The mean coefficient of variation (C.V.) of the DNA content was found to be significantly (P = 0.0002) higher in immature blasts and promyelocytes than in mature granulocytes, lymphocytes, and monocytes. The finding was not due to high DNA contents secondary to DNA-synthesis in immature cells, since the percentage of such cells with a reduced DNA content was also significantly (P = 0.0424-0.0002) increased. The error of the method expressed as the variance of multiple measurements has been found to be 0.0002-0.0004. A staining error has been found for some hydrolysis times, but not for times between 60 and 120 minutes. A measuring error was found for an average of 1.38 to 3.38% of the cells. If many normal cells with DNA-aneuploidy are sterile, this would explain the present findings as well as previous ones about intra-marrow cell death, and also the fact that the variability in DNA content could not be detected with cytogenetic or flow cytometric methods.


Mar 1, 1990·American Journal of Clinical Pathology·T W BauerH S Levin
Jun 1, 1989·Human Pathology·G U AuerO Ahrens
May 1, 1989·Journal of Clinical Pathology·T G HoyA Jacobs
Jan 1, 1988·Leukemia Research·R S VermaM Coleman
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Jun 5, 1986·The New England Journal of Medicine·R E ClarkA Jacobs
Apr 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B H Mayall
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Oct 17, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Pontén
Nov 11, 1999·British Journal of Haematology·S WidellJ M Bennett

Related Concepts

Bone Marrow Cells
DNA, Double-Stranded
Flow Cytometry
Colony-Forming Units, Hematopoietic

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