PMID: 9661813Jul 14, 1998Paper

Variation in IgG1 heavy chain allotype does not contribute to differences in biological activity of two human anti-Rhesus (D) monoclonal antibodies

Immunotechnology : an International Journal of Immunological Engineering
T PatersonM C Carter

Abstract

Pooled human anti-Rhesus D antiserum is currently administered for the prevention of RhD alloimmunization. Increased demand, and decreased supply, of donated pooled antiserum has led to the investigation of the suitability of human monoclonal anti-RhD antibodies for use in its place. However, it is unclear which biological properties of monoclonal antibodies are important for function in RhD-positive foetal red cell clearance and the prevention of alloimmunization. Various antibodies behave differently in a number of in vitro assays of biological function. To compare the function and structure of two human anti-RhD IgG1 monoclonal antibodies which differ in their ability to promote red cell lysis in vitro. In particular to examine whether the functional differences correlate to differences in the IgG1 heavy chain constant region (allotype). We report here the cloning, characterization and re-expression in stable myeloma cell transformants of cDNAs coding for two such antibodies, secreted by the heterohybridoma cell lines ESD-1 (THERAD 03) and LHM 70/45.3 (THERAD 06). The cDNAs were then recombined to exchange portions of the Fc encoding regions and the recombinant antibodies were assayed in vitro to determine RhD-positive red c...Continue Reading

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Citations

Jan 1, 2004·Veterinary Immunology and Immunopathology·Fuqiang Li, Robert Aitken
Mar 14, 2002·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·D L SiegelS L Spitalnik
Jun 15, 2007·Nature Immunology·Masayuki HiranoMax D Cooper
Feb 17, 2007·Transfusion·Leif Kofoed NielsenMorten Hanefeld Dziegiel
May 27, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Leif Kofoed NielsenMorten Hanefeld Dziegiel
Jan 15, 2010·Journal of Health Services Research & Policy·Niina KolehmainenCynthia Fraser
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nidia Alvarez-RuedaStéphane Birklé
Mar 11, 2006·Science·Janardan P Pandey

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