Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion

International Journal of Molecular Sciences
Yong Jin KimChang Suk Suh

Abstract

The expression profile of microRNA (miRNA) in uterine leiomyoma (UL) cells is different from that in normal uterine myometrial (UM) cells. The effect of UL cells on uterine receptivity might vary according to their ability to distort the uterine endometrial cavity. However, the variation in miRNA expression profiles between endometrial cavity-distorting leiomyoma (ECDL) and endometrial cavity non-distorting leiomyoma (ECNDL) cells remains unknown. This study aimed to elucidate whether the expression profile of miRNAs in ECDL cells is dissimilar to that of ECNDL cells in uterus. Pelviscopic myomectomy was performed to obtain tissue samples of UL and their corresponding normal UM tissues (matched) from patients with UL (n = 26), among whom women with ECNDL and ECDL numbered 15 and 11, respectively. The relative expression of hsa-miR-15b, -29a, -29b, -29c, -197, and -200c as well as the candidate target genes in UL cells was compared to those in the matched UM cells using qRT-PCR to assess their ability to cause ECD. The spatial expression of miRNAs and target genes in the UL tissues was analyzed using in situ hybridization. Target gene expression was analyzed using qPCR after transfection with the mimics and inhibitors of miRNAs ...Continue Reading

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Citations

Jun 5, 2019·International Journal of Molecular Sciences·Jae Hoon LeeSiHyun Cho
Nov 21, 2019·Journal of Clinical Laboratory Analysis·Elnaz FazeliReza Mirfakhraie
Dec 7, 2018·International Journal of Molecular Sciences·Michał CiebieraGrzegorz Jakiel
Apr 30, 2020·International Journal of Molecular Sciences·Michał CiebieraArtur Czekierdowski
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Jun 12, 2021·Frontiers in Cell and Developmental Biology·Antonia NavarroAyman Al-Hendy
Dec 30, 2021·Physiology·Elizabeth A Stewart, Romana A Nowak

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Methods Mentioned

BETA
Transfection
PCR

Software Mentioned

SPSS
Statistical Package for the Social Sciences

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