Variation in pollen-donor composition among pollinators in an entomophilous tree species, Castanea crenata, revealed by single-pollen genotyping

PloS One
Yoichi HasegawaKenji Seiwa

Abstract

In plants, reproductive success is largely determined by the composition of pollen (i.e., self-pollen and outcross-pollen from near and distant pollen-donors) transported as a result of pollinator foraging behavior (e.g., pollen carryover). However, little evidence is available on how and to what extent the pollen carryover affects the pollen-donor composition and on which insect taxa are effective outcross-pollen transporters under field conditions. In this study, we explored roles of foraging behavior of insect pollinators on pollen-donor composition and subsequent reproductive success in a woody plant. We performed paternity analyses based on microsatellite genotyping of individual pollen grains found on diurnal pollinators (i.e., bumblebee, small bee, fly, small beetle, and honeybee) visiting Castanea crenata trees. The outcross-pollen rate was highest in bumblebees (66%), followed by small bees (35%), flies (31%), and small beetles (18%). The effective number of pollen donors, representing pollen carryover, was greater in bumblebees (9.71) than in flies (3.40), small bees (3.32), and small beetles (3.06). The high percentages of pollen from outside the plot on bumblebees (65.4%) and flies (71.2%) compared to small bees (35...Continue Reading

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Citations

Apr 17, 2017·Trends in Plant Science·Siegfried L KraussStephen D Hopper
Apr 4, 2017·The New Phytologist·Laura ParducciMikkel Winther Pedersen
May 23, 2019·Annals of Botany·Bronwyn M AyreSiegfried L Krauss
Oct 6, 2019·Annals of Botany·Dorothy A ChristopherJeffrey D Karron
Sep 24, 2020·American Journal of Botany·Bruce Anderson, Corneile Minnaar
Dec 12, 2018·Annals of Botany·Corneile MinnaarJeffrey D Karron
Mar 9, 2017·PloS One·Rashmi Prava MohantyEstelle Levetin
Jun 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Kazuharu OhashiJames D Thomson

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

GeneScan
glmmML
SPAGeDi
CERVUS
R

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