PMID: 9006056Feb 1, 1997Paper

Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli W3110

Journal of Bacteriology
M Jishage, A Ishihama

Abstract

The composition of RNA polymerase sigma subunits was analyzed for stock strains of Escherichia coli K-12 W3110 in Japan. Heterogeneity was discovered with respect to two sigma subunits, sigma28 (sigmaF, the rpoF gene product) and sigma38 (sigmaS, the rpoS gene product). Five different types of W3110 were identified: A-type lineages have both sigma subunits in intact forms; B-type lineages carry a truncated sigma38 subunit and an intact sigma28 subunit; C-type lineages carry an intact sigma28 subunit but lack a sigma38 subunit; D-type lineages have only a sigma38 subunit without a sigma28 subunit; and E-type stocks lack both sigma subunits. All the lineages examined, however, contain the intact forms of sigma70 (sigmaD, the rpoD gene product) and sigma54 (sigmaN, the rpoN gene product). As expected from the lack of a sigma28 subunit, cells of D- and E-type lineages are nonmotile. The truncated form of the sigma38 subunit in B-type stocks carries two mutations near its N terminus and lacks C-terminal proximal region 4 due to an amber mutation. The failure of C- and E-type W3110 cells to express sigma38 and that of D- and E-type cells to express sigma28 were found to be due to defects in transcription even though the respective si...Continue Reading

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Citations

Mar 4, 1999·Journal of Applied Microbiology·A FerreiraK J Boor
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T A AzamA Ishihama
Nov 28, 2001·Environmental Microbiology·T Ferenci
Mar 16, 2005·FEMS Microbiology Letters·Thea King, Thomas Ferenci
Oct 28, 2014·Bioscience, Biotechnology, and Biochemistry·Kaneyoshi YamamotoAkira Ishihama
Nov 7, 2014·PloS One·Yuki YamanakaKaneyoshi Yamamoto
Jul 26, 2015·Science China. Life Sciences·AliAzam Talukder, Akira Ishihama
Apr 16, 2015·Microbial Biotechnology·Emma SevillaFernando Rojo
Nov 1, 2017·FEMS Microbiology Letters·Tomohiro ShimadaAkira Ishihama
May 31, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·G UmitsukiK Nagai
May 3, 2002·European Journal of Biochemistry·Akira KatayamaAkira Ishihama
Mar 20, 2010·FEMS Microbiology Reviews·Juana María Navarro LlorensEsteban Martínez-García
Nov 27, 2007·Molecular Microbiology·Fanette FontaineFrançois Taddei
Oct 31, 2013·Scientific Reports·Tadayuki IwaseYoshimitsu Mizunoe
Dec 31, 2015·PloS One·Michihisa MaedaAkira Ishihama
May 12, 2017·Frontiers in Microbiology·Tadayuki IwaseYoshimitsu Mizunoe
Mar 13, 2018·Nucleic Acids Research·Tomohiro ShimadaAkira Ishihama
Jan 21, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Makoto MiyataKen-Ichi Wakabayashi
Dec 13, 2006·Genes & Genetic Systems·Akira NakagawaHirotada Mori
May 9, 2014·Future Microbiology·Herb E Schellhorn
Oct 24, 2013·Environmental Microbiology·Esteban Martínez-GarcíaVíctor de Lorenzo
May 28, 2014·Archives of Microbiology·Heloisa F GalbiatiBeny Spira
Sep 15, 2017·The Journal of General and Applied Microbiology·Akira Ishihama
Feb 29, 2020·Scientific Reports·Yukari Miyake, Kaneyoshi Yamamoto

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