Variation of expression defects in cell surface 190-kDa protein antigen of Streptococcus mutans

International Journal of Medical Microbiology : IJMM
Jinthana LapirattanakulKazuhiko Nakano

Abstract

Streptococcus mutans, which consists of four serotypes, c, e, f, and k, possesses a 190-kDa cell surface protein antigen (PA) for initial tooth adhesion. We used Western blot analysis to determine PA expression in 750 S. mutans isolates from 150 subjects and found a significantly higher prevalence of the isolates with PA expression defects in serotypes f and k compared to serotypes c and e. Moreover, the defect patterns could be classified into three types; no PA expression on whole bacterial cells and in their supernatant samples (Type N1), PA expression mainly seen in supernatant samples (Type N2), and only low expression of PA in the samples of whole bacterial cells (Type W). The underlying reasons for the defects were mutations in the gene encoding PA as well as in the transcriptional processing of this gene for Type N1, defects in the sortase gene for Type N2, and low mRNA expression of PA for Type W. Since cellular hydrophobicity and phagocytosis susceptibility of the PA-defective isolates were significantly lower than those of the normal expression isolates, the potential implication of such defective isolates in systemic diseases involving bacteremia other than dental caries was suggested. Additionally, multilocus seque...Continue Reading

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Citations

Mar 19, 2016·Molecular Oral Microbiology·A Avilés-ReyesJ Abranches
Jan 1, 2016·Nanomaterials·Ángel Manuel Martínez-RoblesLeón Francisco Espinosa-Cristóbal

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