Variations in platelet protein associated with arterial thrombosis

Thrombosis Research
Elena G Arias-SalgadoMatilde S Ayuso

Abstract

Hyperactivity of platelets has been associated with thrombotic episodes by molecular mechanisms not yet elucidated. The present work aimed at identifying whether the platelet protein content from patients who had suffered an arterial thrombosis episode differed from that of platelets obtained from normal healthy donors. Differential platelet protein profiles were determined by 2-dimensional (2-D) gel electrophoresis and Western blot analysis of total platelet lysates. Identification of differentially expressed proteins was carried out by mass spectrometry (MALDI-TOF). We found a decreased platelet content of three protein spots in patients of arterial thrombosis: integrin linked kinase (ILK), fructose bisphosphate aldolase (aldolase) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) whereas the content of four other protein spots was increased: actin binding protein, coronine like (p57), non-muscle myosin heavy chain (NMMHC-A), pyruvate kinase M2 isoenzyme (PK) and phosphoglycerate kinase (PGK). The variations in ILK, GAPDH and PK were validated by Western blot analysis. The proteins showing a decreased platelet content in arterial thrombosis patients are associated with the cytoskeletal insoluble fraction and the detected i...Continue Reading

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Citations

Apr 15, 2014·TheScientificWorldJournal·Kakali Ghoshal, Maitree Bhattacharyya
Feb 26, 2013·Journal of Proteomics·Ivano EberiniElisabetta Gianazza
Oct 20, 2011·Mass Spectrometry Reviews·Anne ZuffereyJean-Charles Sanchez
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·S MarconeD J Fitzgerald
Mar 8, 2016·Proteomics. Clinical Applications·Ángel García
Oct 11, 2016·Expert Review of Proteomics·Irene Izquierdo, Ángel García
Mar 7, 2019·Journal of Cellular and Molecular Medicine·Michal BijakJoanna Saluk-Bijak
Jul 8, 2020·International Journal of Molecular Sciences·Erica GianazzaElena Tremoli
May 6, 2021·International Journal of Molecular Sciences·Olga ShevchukAlbert Sickmann

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