PMID: 3768372Nov 7, 1986Paper

Variations in the glutathione S-transferase subunits expressed in human livers

Biochimica Et Biophysica Acta
A J HusseyJ D Hayes

Abstract

Human livers express a variety of cytosolic glutathione S-transferase isoenzymes. The enzymes are subject to a marked polymorphism and the polypeptide basis of the differences in glutathione S-transferase content of individual livers has been investigated by Western blotting, hydroxyapatite HPLC and isoelectric focusing. Collectively, the livers examined contained three distinct groups of cytosolic glutathione S-transferase. The three classes of enzyme contain subunits of different molecular mass; subunits of 24.8 kDa (Yf), 26.0 kDa (Ya) and 26.7 kDa (Yb) were found to belong to the 'acidic-type', 'basic-type' and 'neutral-type' glutathione S-transferase, respectively. All livers studied contained 26.0 kDa subunits (Ya or 'basic') but significant differences in the isoelectric points of this group of proteins were demonstrated. Five of the eight livers examined expressed 26.7 kDa subunits (Yb or 'neutral'); the native enzymes had pI values of either 6.1 or 5.5, and were isolated by hydroxyapatite HPLC. Two of the livers possessed 24.8 kDa subunits (Yf or 'acidic'), and the native enzyme, which had a pI of 4.8, was also purified by hydroxyapatite HPLC. Before undertaking a glutathione S-transferase purification it is advisable t...Continue Reading

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Citations

Feb 5, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C LiY Zhou
Jan 1, 1990·Chemico-biological Interactions·R M Vos, P J Van Bladeren
Jul 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·N TateokaK Sato
Aug 1, 1990·Gut·D J HarrisonJ D Hayes
Nov 1, 1995·Human & Experimental Toxicology·J D Hayes
Jan 1, 1988·CRC Critical Reviews in Biochemistry·B Mannervik, U H Danielson
Jul 1, 1988·Archives of Biochemistry and Biophysics·S V SinghY C Awasthi
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·B Coles, B Ketterer
Oct 8, 1987·Biochimica Et Biophysica Acta·K D TewT M Smith
Oct 1, 1990·Biochemical Medicine and Metabolic Biology·N OzerI Ozer

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