May 1, 1971

Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells

Journal of Bacteriology
H P Klein, L Jahnke

Abstract

In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Chloride Ion Level
Centrifugation, Density Gradient
Cytochrome c Oxidase Subunit VIa
Dioxygen
Saccharomyces cerevisiae allergenic extract
Succinate Dehydrogenase
Microsomes
Acetyl-coenzyme A Synthetase
Saccharomyces

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