Variations of Elastic Modulus and Cell Volume with Temperature for Cortical Neurons

Langmuir : the ACS Journal of Surfaces and Colloids
Jacob P SunnerbergCristian Staii

Abstract

Neurons change their growth dynamics and mechanical properties in response to external stimuli such as stiffness of the local microenvironment, ambient temperature, and biochemical or geometrical guidance cues. Here we use combined atomic force microscopy (AFM) and fluorescence microscopy experiments to investigate the relationship between external temperature, soma volume, and elastic modulus for cortical neurons. We measure how changes in ambient temperature affect the volume and the mechanical properties of neuronal cells at both the bulk (elastic modulus) and local (elasticity maps) levels. The experimental data demonstrate that both the volume and the elastic modulus of the neuron soma vary with changes in temperature. Our results show a decrease by a factor of 2 in the soma elastic modulus as the ambient temperature increases from room (25 °C) to physiological (37 °C) temperature, while the volume of the soma increases by a factor of 1.3 during the same temperature sweep. Using high-resolution AFM force mapping, we measure the temperature-induced variations within different regions of the elasticity maps (low and high values of elastic modulus) and correlate these variations with the dynamics of cytoskeleton components an...Continue Reading

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Citations

Nov 26, 2019·Soft Matter·Joao Marcos Vensi BassoCristian Staii
Jul 3, 2021·Biomimetics·Ilya YurchenkoCristian Staii
Sep 24, 2021·PloS One·Jacob P SunnerbergCristian Staii

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