Variations of gastric corpus microbiota are associated with early esophageal squamous cell carcinoma and squamous dysplasia

Scientific Reports
D NasrollahzadehWeimin Ye

Abstract

Observational studies revealed a relationship between changes in gastric mucosa and risk of esophageal squamous cell carcinoma (ESCC) which suggested a possible role for gastric microbiota in ESCC carcinogenesis. In this study we aimed to compare pattern of gastric corpus microbiota in ESCC with normal esophagus. Cases were included subjects with early ESCC (stage I-II) and esophageal squamous dysplasia (ESD) as the cancer precursor. Control groups included age and sex-matched subjects with mid-esophagus esophagitis (diseased-control), and histologically normal esophagus (healthy-control). DNA was extracted from snap-frozen gastric corpus tissues and 16S rRNA was sequenced on GS-FLX Titanium. After noise removal, an average of 3004 reads per sample was obtained from 93 subjects. We applied principal coordinate analysis to ordinate distances from beta diversity data. Pattern of gastric microbiota using Unifrac (p = 0.004) and weighted Unifrac distances (p = 0.018) statistically varied between cases and healthy controls. Sequences were aligned to SILVA database and Clostridiales and Erysipelotrichales orders were more abundant among cases after controling for multiple testing (p = 0.011). No such difference was observed between m...Continue Reading

References

Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P Boyle
Sep 1, 1973·British Journal of Cancer·E MahboubiS Salmasizadeh
Jan 23, 1998·Scandinavian Journal of Gastroenterology·J H SimánC H Florén
Mar 31, 2000·European Journal of Gastroenterology & Hepatology·F VianiD Armstrong
Oct 12, 2000·British Journal of Cancer·F SaidiM K Roshan
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R G QuiveyK Hahn
May 7, 2002·Clinical Medicine : Journal of the Royal College of Physicians of London·Barry Marshall
Nov 8, 2003·World Journal of Gastroenterology : WJG·Ke-Xia WangChao-Pin Li
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Mar 4, 2005·The American Journal of Gastroenterology·Deng-Chyang WuMing-Tsang Wu
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth M BikDavid A Relman
Oct 31, 2006·Applied and Environmental Microbiology·Rebecca J CaseStaffan Kjelleberg
Dec 21, 2006·British Journal of Cancer·F KamangarS M Dawsey
Mar 30, 2007·Journal of Microbiological Methods·Soumitesh ChakravortyDavid Alland
May 10, 2007·The American Journal of Gastroenterology·Katsunori IijimaTooru Shimosegawa
Jul 31, 2007·Genome Biology·Susan M HuseDavid Mark Welch
Nov 8, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Christian C AbnetSanford M Dawsey
Dec 25, 2008·International Journal of Cancer. Journal International Du Cancer·Annemarie C de VriesErnst J Kuipers
Feb 1, 2011·BMC Bioinformatics·Christopher QuincePeter J Turnbaugh
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Apr 8, 2011·The ISME Journal·Daniel Pr HerlemannAnders F Andersson
May 5, 2012·Bioinformatics·Elmar PruesseFrank Oliver Glöckner
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Apr 4, 2013·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Philip R TaylorSanford M Dawsey
Jun 14, 2013·Environmental Microbiology Reports·Daniel LundinEva S Lindström
Dec 10, 2013·Journal of the National Cancer Institute·Jiyoung AhnLiying Yang
Apr 5, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Guoqin YuChristian C Abnet

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Citations

Jan 6, 2016·British Journal of Cancer·Emily Vogtmann, James J Goedert
Sep 16, 2015·Helicobacter·Gianluca IaniroAntonio Gasbarrini
Jun 19, 2015·Clinical and Translational Gastroenterology·Parth J ParekhDavid A Johnson
Oct 28, 2016·Annals of the New York Academy of Sciences·Vincenzo Di PilatoPaolo Bechi
Jan 8, 2017·European Journal of Epidemiology·Hans-Olov Adami, Olof Nyrén
Jun 25, 2017·Carcinogenesis·Marco MatejcicPietro Ferrari
Jun 21, 2018·Journal of Medical Microbiology·Yanfang LiuZhijian Hu
Apr 10, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Concetta PanebiancoValerio Pazienza
Dec 21, 2018·PLoS Pathogens·Teminioluwa A AjayiKatherine S Garman
Jan 20, 2018·Current Treatment Options in Gastroenterology·Michael May, Julian A Abrams
Feb 15, 2020·Alimentary Pharmacology & Therapeutics·Mirjana Rajilic-StojanovicGeorgina L Hold
Mar 4, 2020·International Journal of Molecular Sciences·Caterina VivaldiLorenzo Fornaro
Jul 16, 2020·Gastroenterology Report·Xiaoli Zhang, Zui Pan
Jun 4, 2020·Genome Biology·Sumeed Syed ManzoorMichael B Burns
Jun 19, 2020·Journal of Diabetes and Metabolic Disorders·Hossein AazamiBagher Larijani
Jul 18, 2020·Frontiers in Cellular and Infection Microbiology·Donghang LiQing Geng
Jun 5, 2018·Annals of Gastroenterological Surgery·Yoshifumi BabaHideo Baba
May 23, 2020·Clinical and Experimental Gastroenterology·Lina ElsalemOsama Aloudat
Jul 12, 2019·Frontiers in Cellular and Infection Microbiology·Pengjun ZhouYifei Wang
Nov 18, 2020·Cellular Oncology (Dordrecht)·Miao-Fen ChenWen-Cheng Chen
Jul 31, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Inge HuybrechtsNathalie Michels
Jul 22, 2020·Seminars in Cancer Biology·Var Ruchi SharmaAnil K Sharma
Feb 24, 2018·Genomics, Proteomics & Bioinformatics·Changting MengQiang Tian
May 27, 2021·Applied Microbiology and Biotechnology·Changchang ChenJianxin Lyu
Sep 16, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G MurphyP Brennan
Jun 14, 2021·Seminars in Cancer Biology·Muhammad Jameel Mughal, Hang Fai Kwok
Jul 9, 2021·Digestive Diseases and Sciences·Ali SoroushJulian A Abrams
Aug 21, 2021·BMC Cancer·Zi-Kun YuPei-Yu Huang
Aug 28, 2021·Microorganisms·Yukiko YanoChristian C Abnet
Dec 9, 2020·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Jotamo ComeLúcio Lara Santos

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Methods Mentioned

BETA
biopsy
454 sequencing
biopsies
PCR
electrophoresis

Software Mentioned

R
phyloseq package
SINA aligner
FCluster
AmpliconNoise

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