Variations on a theme: evolution of the phage-shock-protein system in Actinobacteria

Antonie van Leeuwenhoek
Janani RaviMaria Laura Gennaro

Abstract

The phage shock protein (Psp) stress-response system protects bacteria from envelope stress through a cascade of interactions with other proteins and membrane lipids to stabilize the cell membrane. A key component of this multi-gene system is PspA, an effector protein that is found in diverse bacterial phyla, archaea, cyanobacteria, and chloroplasts. Other members of the Psp system include the cognate partners of PspA that are part of known operons: pspF||pspABC in Proteobacteria, liaIHGFSR in Firmicutes, and clgRpspAMN in Actinobacteria. Despite the functional significance of the Psp system, the conservation of PspA and other Psp functions, as well as the various genomic contexts of PspA, remain poorly characterized in Actinobacteria. Here we utilize a computational evolutionary approach to systematically identify the variations of the Psp system in ~450 completed actinobacterial genomes. We first determined the homologs of PspA and its cognate partners (as reported in Escherichia coli, Bacillus subtilis, and Mycobacterium tuberculosis) across Actinobacteria. This survey revealed that PspA and most of its functional partners are prevalent in Actinobacteria. We then found that PspA occurs in four predominant genomic contexts wi...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J L BrissetteP Model
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 21, 1998·Nucleic Acids Research·E L SonnhammerR Durbin
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Jul 21, 2004·Briefings in Bioinformatics·Igor B RogozinEugene V Koonin
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Thorsten MascherJohn D Helmann
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Jul 28, 2005·Molecular Microbiology·Andrew J Darwin
Nov 21, 2007·Methods in Molecular Biology·Jaina Mistry, Robert Finn
Mar 11, 2008·Journal of Bacteriology·Kristof VranckenJozef Anné
May 9, 2008·Nucleic Acids Research·Christian ColeGeoffrey J Barton
Oct 25, 2008·Nucleic Acids Research·Eugene V Koonin, Yuri I Wolf
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Dec 2, 2011·Journal of Experimental Botany·Ute C VothknechtDirk Schneider
Nov 30, 2012·Nucleic Acids Research·Dennis A BensonEric W Sayers
Apr 4, 2013·BMC Genomics·Lex OvermarsChristof Francke
Nov 15, 2013·Nucleic Acids Research·Alice R WattamBruno W Sobral
Jun 15, 2016·Annual Review of Microbiology·Josué Flores-Kim, Andrew J Darwin
Apr 30, 2017·Methods in Molecular Biology·Henrik Nielsen

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Software Mentioned

PATRIC
R
BLAST
FastTree
BLASTCLUST
Kalign
PSIBLAST

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