PMID: 11928988Apr 4, 2002Paper

Varicella-zoster virus (VZV) mediates a delayed host shutoff independent of open reading frame (ORF) 17 expression

Virus Genes
Tim WaterboerManfred H Wolff

Abstract

Varicella-zoster virus (VZV) open reading frame 17 (ORF 17) is the gene corresponding to Herpes simplex-virus (HSV) UL41. The UL41 gene encodes the virion host shutoff factor (vhs), a RNase that has been the object of detailed studies. In contrast to HSV, knowledge about VZV mediated shutoff effects and the role of ORF 17 is poor. We investigated the ORF 17 expression in infected cells and analyzed shutoff effects. ORF 17 expression could not be proven in infected human fibroblast cell lines and melanoma (MeWo) cells. Only after induction by Phorbol 12-myristate 13-acetate an ORF 17 expression became detectable in MeWo cells. Nevertheless, using stable expressed GAPDH mRNA as a marker for mRNA degradation, a VZV mediated shutoff, independent of ORF 17 expression, became measurable. Transfection experiments demonstrated that transient ORF 17 expression did not decrease the cellular GAPDH mRNA level. We examined whether the VZV shutoff factor is a tegument protein causing an early shutoff or whether it needs to be expressed (delayed shutoff). The GAPDH mRNA level in Actinomycin D pretreated and infected MeWo cells did not decrease even faster than the theoretical decay rate based on a half-life of 24 h. These findings lead to the...Continue Reading

Citations

Mar 11, 2006·The Journal of General Virology·Markus RahausManfred H Wolff
Jun 24, 2010·Archives of Virology·Mandar S PaingankarDileep N Deobagkar
Dec 29, 2013·Journal of Virology·Brett A DuguayJames R Smiley
Nov 17, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Patricia B RozenchanM Mitzi Brentani

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