Varied persistent life cycles of Borna disease virus in a human oligodendroglioma cell line

Journal of Virology
Madiha S IbrahimKazuyoshi Ikuta

Abstract

Borna disease virus (BDV) establishes a persistent infection in the central nervous system of vertebrate animal species as well as in tissue cultures. In an attempt to characterize the life cycle of BDV in persistently infected cultured cells, we developed 30 clones by single-cell cloning from a human oligodendroglioma (OL) cell line after infection with BDV. According to the percentage of cells expressing the BDV major proteins, p40 (nucleoprotein) and p24 (phosphoprotein), the clones were classified into two types: type I (>20%) and type II (<20%). mRNAs corresponding to both proteins were detected by in situ hybridization (ISH) in a percentage of cells consistent with that for the protein expression in the two types. Surprisingly, ISH for the detection of the genomic RNA, mainly in type II, revealed a significantly larger cell population harboring the genomic RNA than that with the protein as well as the mRNA expression. By recloning from type II primary cell clones, the same phenotype was confirmed in the secondary cell clones obtained: i.e., low percentage of protein-positive cells and higher percentage of cells harboring the genomic RNA. After nerve growth factor treatment, the two types of clones showed increases in the ...Continue Reading

References

Jan 1, 1979·Archives of Virology·K Danner, A Mayr
May 1, 1976·Infection and Immunity·K ShimokataI Nagata
Nov 11, 1991·Journal of Virology·K M CarboneG B Vogelsang
Jan 1, 1990·Progress in Brain Research·M Nieto-Sampedro, P Bovolenta
Aug 1, 1990·Virology·S Schneider-SchauliesV ter Meulen
Dec 1, 1989·Biological Psychiatry·W DittrichK Schneider
Jul 1, 1988·The Journal of Infectious Diseases·K BaczkoV ter Meulen
Dec 1, 1982·The Journal of General Virology·S Jacobson, H F McFarland
Jan 1, 1995·Acta Neuropathologica·C F MüllerA Zurbriggen
Jan 1, 1995·Current Topics in Microbiology and Immunology·R Rott, H Becht
Mar 1, 1994·Journal of Virology·B CubittJ C de la Torre
Aug 1, 1994·Journal of Virology·P A SchneiderW I Lipkin
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·T BrieseW I Lipkin
Jan 1, 1993·Archives of Virology. Supplementum·L BodeG Czech
Jun 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A L LundgrenH Ludwig
Sep 1, 1993·The Journal of General Virology·M MarschallH Meier-Ewert
Dec 21, 1995·Brain Research. Developmental Brain Research·J R BautistaK M Carbone
Oct 27, 1997·The Journal of Biological Chemistry·J A Recio, A Aranda
Feb 7, 1998·Acta Neuropathologica·A ZurbriggenM Vandevelde
Aug 24, 1999·The Journal of Veterinary Medical Science·T MizutaniI Takashima
Apr 25, 2000·Journal of Virology·Y NakamuraK Ikuta

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