PMID: 9523844Apr 2, 1998Paper

Variegated transfer of recombinant glycosylphosphatidylinositol-anchored CD4 among cultured cells: correlation of flow cytometric and microscopic observations

The Journal of Laboratory and Clinical Medicine
J L MillerN K Dwyer

Abstract

Glycosylphosphatidylinositol-anchored proteins (GPI-proteins) expressed on the outer leaflet of cell membranes are involved in diverse physiologic as well as pathologic processes in humans. Previously, we demonstrated the intercellular transfer of overexpressed CD4-GPI in vitro from transduced HeLa cells to their parental cell line. In this report we present further information on the transfer process and the nature of the transferred GPI-proteins. In mixed-cell populations, the transfer of CD4-GPI was detectable within minutes at levels proportional to the ratio of donor and recipient cells. The amount of CD4-GPI detected with flow cytometry on the surface of the recipient cells varied according to cell type. Microscopy of mixed cell populations revealed discrete CD4-GPI containing aggregates on the target cells, whereas colocalized transfer of cytoplasm was not detected. Separation of cocultivated cells by semipermeable membranes largely prevented CD4-GPI transfer, but aggregates containing CD4-GPI were demonstrated by electron microscopy in supernatants passed through filters of 0.4-mm pore size.

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Citations

Dec 22, 1999·Human Immunology·Z G FridlenderM Schlesinger
Apr 2, 1998·The Journal of Laboratory and Clinical Medicine·M G Low
Oct 3, 2002·The Journal of Biological Chemistry·Tong LiuMan Sun Sy

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