Variety of angiotensin receptors in 3T3-L1 preadipose cells and differentiated adipocytes

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
F Weiland, E J Verspohl

Abstract

A local paracrine acting angiotensin (ANG) system of preadipocytes and mature adipocytes is involved in metabolic effects and tissue differentiation. The present study reports on the investigation of binding affinities for various angiotensin receptors including their relevance in 3T3-L1 adipocytes and preadipocytes and 3T3-442A preadipocytes. Competitive binding studies using both 125I-ANG II and its more stable analogue 125I-SARILE for investigating AT1/AT2 binding sites in 3T3-L1 preadipocytes reveal a biphasic competition curve with KDs at a low and high nanomolar range. By using the AT2 receptor selective ligand 125I-CGP4112A the presence of high affinity AT2 binding sites in preadipocytes was observed. High nonspecific binding and a low receptor number is characteristic for all these experiments. An AT4 binding site (binding site for ANG IV) exists in 3T3-L1 and F442A preadipocytes and adipocytes with a high nanomolar KD. This low binding affinity was confirmed by a biological assay, the IRAP assay (=insulin regulated aminopeptidase assay). IRAP is associated with the AT4 receptor, which is a binding site at the luminal part of membrane bound IRAP. The curves for competition binding and for inhibition of IRAP activity are...Continue Reading

Citations

Apr 12, 2012·Pflügers Archiv : European journal of physiology·Justin L GrobeCurt D Sigmund
Jul 4, 2012·Molecular and Cellular Endocrinology·Yonit MarcusNaftali Stern
Oct 15, 2010·Kidney International·Laurent Yvan-Charvet, Annie Quignard-Boulangé
Oct 1, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Felix Weiland, Eugen J Verspohl
Apr 8, 2014·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Ilse A C ArnoldussenDeborah R Gustafson
Oct 15, 2013·The Journal of Nutritional Biochemistry·Maria E FrigoletArmando R Tovar
Feb 18, 2014·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Alexandros NikolaouJo A Van Ginderachter
Mar 7, 2014·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Arif U HasanMasakazu Kohno
Jan 10, 2019·International Journal of Molecular Sciences·Myoungsook LeeInhae Kang
Jun 8, 2010·Journal of Receptor and Signal Transduction Research·M SiebelmannE J Verspohl

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