PMID: 2126278Dec 1, 1990Paper

Varying dose-response characteristics of different immunoassays and an in-vitro bioassay for FSH are responsible for changing ratios of biologically active to immunologically active FSH

The Journal of Endocrinology
F JockenhövelE Nieschlag

Abstract

Serum FSH levels in fertile and infertile men were determined by applying the Sertoli cell in-vitro bioassay and six different immunoassays. Bioassay and immunoassay estimates were significantly correlated (r ranging from 0.78 to 0.86; P less than 0.01). On average, all immunoassays measured lower FSH concentrations in samples with low FSH levels and higher FSH concentrations in those with high FSH levels compared with the bioassay. Ratios of bioactivity to immunoreactivity (B/I) were highest in fertile men and lowest in men with severe disturbances of testicular function. Depending on which immunoassay was used these differences were either significant or only marginal. Dose-response characteristics for WHO FSH standard preparation 78/549, used in the bioassay as well as in the immunoassays, were different between immunoassays and the bioassay, suggesting that decreasing B/I ratios with increasing FSH serum levels were method-related and reflected different slopes of the dose-response characteristics of the assays, rather than being true changes in the molecular composition of FSH. The present investigation underlines the necessity of choosing the immunoassay used for comparison with the bioassay carefully and of validating th...Continue Reading

Citations

Feb 1, 1992·Cell and Tissue Research·M BergmannE Nieschlag
Jan 1, 1994·Clinical Endocrinology·M BergmannE Nieschlag
Sep 1, 1991·Clinical Endocrinology·A TsatsoulisW R Robertson
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Dec 1, 1991·Journal of Endocrinological Investigation·M Simoni, E Nieschlag
Sep 1, 1992·Annals of Clinical Biochemistry·M A PollockC B Winnard
Jul 10, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·M P Rose
Oct 20, 2006·Journal of Chromatography. a·Renan Fernandes LoureiroMaria Teresa C P Ribela

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