PMID: 12788388Jun 6, 2003Paper

Varying susceptibility to myocardial infarction among C57BL/6 mice of different genetic background

Journal of Molecular and Cellular Cardiology
Diana A GorogMichael S Marber

Abstract

Genetically manipulated mouse lines are invaluable to investigate the effects of a single gene on sensitivity to ischemia. When choosing appropriate controls, we were concerned that intrinsic, strain-independent but colony-dependent differences may influence the susceptibility to ischemia. We, therefore, compared the infarct:risk volume ratio (I:R%) after 30-min global ischemia in Langendorff-perfused hearts from outbred C57BL/6 mice with that in wild-type mice derived from heterozygote x heterozygote crosses of two different in-house C57BL/6 mouse lines with targeted disruption of an MKK3 or MAPKAPK2 allele. Despite similar hemodynamic characteristics, I:R% in outbred C57BL/6 hearts was significantlysmaller (40.8 +/- 2.8%) than in C57BL/6 MAPKAPK2 wild types (65.8 +/- 4.5%, P = 0.0003) and significantly larger than in C57BL/6 MKK3 wild types (23.7 +/- 2.9%, P = 0.002). Therefore, inherent colony substrain-dependent differences appear to influence the susceptibility to infarction in response to global ischemia, underscoring the importance of using colony-matched wild-type controls in murine studies of myocardial ischemia.

Citations

May 21, 2011·Basic Research in Cardiology·Alexander SirkerAjay M Shah
Apr 15, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ibrahim AbdullahPeter J Gruber
Nov 3, 2012·Experimental Physiology·Adelaide GrecoAlberto Cuocolo
Oct 16, 2012·Experimental Physiology·James E Clark, Michael S Marber
Aug 30, 2008·Experimental Physiology·Melissa E ReicheltJohn P Headrick
Apr 17, 2012·Journal of Biomedicine & Biotechnology·Sara GargiuloAlberto Cuocolo
Aug 1, 2006·Journal of the American College of Cardiology·Mohamed BellahceneMichael S Marber
Jan 30, 2007·Journal of Molecular and Cellular Cardiology·Kevin J AshtonJohn P Headrick
Aug 15, 2014·PloS One·Constantin RüderDietlind Zohlnhöfer
Dec 21, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Liang ZhaoThaddeus S Nowak
May 23, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Alamgir M N KabirMichael S Marber
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ming ZhangMichael C Carroll
Aug 19, 2018·Basic Research in Cardiology·Hans Erik BøtkerGerd Heusch
Feb 26, 2021·Journal of Bioenergetics and Biomembranes·Zhou ZhouQinqiang Long

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