Vascular differentiation from pluripotent stem cells in 3-D auxetic scaffolds

Journal of Tissue Engineering and Regenerative Medicine
Liqing SongYan Li

Abstract

Auxetic scaffolds, that is, scaffolds that can display negative Poisson's ratio, have unique physical properties and can expand transversally when axially strained or contract under compression. Auxetic materials have been used for bioprostheses and artery stents due to the enhanced compressive strength and shear stiffness. In vascular tissue engineering, auxetic scaffolds allow the widening of blood vessels when blood flows through (creating compressive stress) to prevent the blockage. However, the influence of auxetic materials on the cellular fate decision in local environment is unclear. In this study, auxetic polyurethane foams were used to support vascular differentiation from pluripotent stem cells. The expression of alkaline phosphatase, Oct-4, and Nanog was lower after 4 days of differentiation for the cells grown in auxetic scaffolds. Higher expression of vascular markers CD31 and VE-cadherin was observed for the cells from auxetic scaffolds compared with those from the scaffolds before auxetic conversion. Little influence on the expression of cardiac marker α-actinin was observed. The vascular cells secreted extracellular matrix proteins vitronectin and laminin and expressed membrane-bound matrix metalloproteinase 9....Continue Reading

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Citations

May 6, 2019·Cells·Taha AzadXiaolong Yang
Mar 28, 2020·Biomaterials Science·Paul MardlingChristine Lyn Le Maitre
Aug 17, 2021·Computer Methods in Biomechanics and Biomedical Engineering·Sundeep Singh, Roderick Melnik

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