Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury

Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
Garrett G MuramotoJohn P Chute

Abstract

The risk of terrorism with nuclear or radiologic weapons is considered to be high over the coming decade. Ionizing radiation can cause a spectrum of hematologic toxicities, from mild myelosuppression to myeloablation and death. However, the potential regenerative capacity of human hematopoietic stem cells (HSCs) after radiation injury has not been well characterized. In this study, we sought to characterize the effects of ionizing radiation on human HSCs and to determine whether signals from vascular endothelial cells could promote the repair of irradiated HSCs. Exposure of human bone marrow CD34+ cells to 400 cGy caused a precipitous decline in hematopoietic progenitor cell content and primitive cells capable of repopulating nonobese diabetic/severe combined immunodeficient mice (SCID-repopulating cells), which was not retrievable via treatment with cytokines. Conversely, culture of 400 cGy-irradiated bone marrow CD34+ cells with endothelial cells under noncontact conditions supported the differential recovery of both viable progenitor cells and primitive SCID-repopulating cells. These data illustrate that vascular endothelial cells produce soluble factors that promote the repair and functional recovery of HSCs after radiation...Continue Reading

References

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Citations

May 6, 2009·In Vivo·Joel S Greenberger
Jul 9, 2016·Radiation Research·Yingying WangDaohong Zhou
Apr 13, 2017·Journal of Radiation Research·Fang ChenJunping Wang
Jun 13, 2017·Radiation Research·Andrea L DiCarloJean-Rene Jourdain
Sep 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Carmen Mariana Aanei, Lydia Campos Catafal
Jan 30, 2020·International Journal of Molecular Sciences·Abhishek K Singh, Jose A Cancelas
Mar 16, 2017·Stem Cells Translational Medicine·Jennifer L GoriHans-Peter Kiem
Feb 14, 2017·Stem Cells Translational Medicine·Joshua P SasineJohn P Chute

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