Vascular endothelial growth factor activates neural stem cells through epidermal growth factor receptor signal after spinal cord injury

CNS Neuroscience & Therapeutics
Su-Mei LiuJian-Wu Dai

Abstract

Neural stem cells (NSCs) in the adult mammalian spinal cord are activated in response to spinal cord injury (SCI); however, mechanisms modulating this process are not clear. Here, we noticed SCI elevated expression of vascular endothelial growth factor (VEGF) and we aimed to validate the roles of VEGF in NSCs activation after SCI and investigated the related signals during the process. In vitro we detected whether VEGF promoted spinal cord NSCs proliferation and investigated the involved signals; In vivo, we injected VEGF into rat spinal cord to check the NSCs activation. In vitro, VEGF triggered spinal cord NSCs proliferation and maintained self-renewal. Further investigations demonstrated VEGF transactivated epidermal growth factor receptor (EGFR) through VEGF receptor 2 (VEGFR2) to promote spinal cord NSCs proliferation. In vivo, we injected VEGF into spinal cord by laminectomy to confirm the roles of VEGF-VEGFR2-EGFR signals in NSCs activation. VEGF significantly elevated the number of activated NSCs and increased EGFR phosphorylation. In contrast, intraspinal injection of specific inhibitors targeting EGFR and VEGFR2 decreased NSCs activation after SCI. Our results demonstrate that VEGF-VEGFR2-EGFR axis is important for NS...Continue Reading

References

Apr 4, 1997·Science·R McKay
Aug 15, 1997·Journal of Neuroscience Research·E PapavassiliouM J Merrill
Nov 30, 1999·Trends in Pharmacological Sciences·E ZwickA Ullrich
Oct 6, 2000·The Journal of Clinical Investigation·Z G ZhangM Chopp
Nov 2, 2001·Nature·S Temple
Aug 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kunlin JinDavid A Greenberg
Sep 3, 2002·Journal of Neuroscience Research·Yoshiki YagitaMasayasu Matsumoto
Sep 3, 2003·Nature Reviews. Genetics·Peter Carmeliet
Dec 31, 2003·Molecular and Cellular Neurosciences·John A BoockvarDonald M O'Rourke
Feb 24, 2004·Journal of Hematotherapy & Stem Cell Research·Daniele BottaiL Angelo Vescovi
Feb 14, 2006·Current Alzheimer Research·Matthew J During, Lei Cao
Feb 7, 2008·Nature Reviews. Neuroscience·Serena ZacchignaPeter Carmeliet
Jul 3, 2008·Cell Stem Cell·Fanie Barnabé-Heider, Jonas Frisén
Aug 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eri Segi-NishidaRonald S Duman
Oct 5, 2010·Organogenesis·Jeffrey M RosensteinChristiana Ruhrberg
Feb 5, 2013·Experimental Neurology·Hanna SabelströmJonas Frisén
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth D KirbyTony Wyss-Coray
Jul 16, 2016·Mediators of Inflammation·Elisa GarciaAntonio Ibarra

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Citations

Feb 28, 2020·Experimental and Therapeutic Medicine·Qingfa ChenFabin Han
Jun 14, 2019·Stem Cells International·Sumei Liu, Zhiguo Chen
Sep 29, 2020·Frontiers in Neuroscience·Pieter VancampSylvie Remaud
Aug 19, 2020·Cells·Roberta Romano, Cecilia Bucci
Sep 26, 2020·Journal of Cellular and Molecular Medicine·Xin WangTianyi Wang

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