Vascular endothelial growth factor aggravates cerebral ischemia and reperfusion-induced blood-brain-barrier disruption through regulating LOC102640519/HOXC13/ZO-1 signaling

Experimental Cell Research
Li WuQingchun Gao

Abstract

Vascular endothelial growth factor (VEGF) has been recognized to be a potential pharmaceutical target for treating ischemic stroke, but its severe side effects hinder its widely application. Here, the present study was designed to investigate the effects of VEGF on blood-brain-barrier (BBB) disruption and the underlying mechanisms. A mouse model of middle cerebral artery occlusion (MCAO) was constructed and treated with or without VEGF. Meanwhile, mice brain microvascular endothelial cells in co-culture with astrocytes were subjected to 1, 2 and 4 h oxygen-glucose deprivation followed by 24 h of reperfusion (OGD/R) in the absence or presence of VEGF. The mRNA and protein expression were assessed by real-time PCR and Western blotting. Fluorescence in situ hybridization (FISH) was utilized to validate LOC102640519 expression in OGD/R cell models. Chromatin Immunoprecipitation (ChIP) assay was used to confirm the regulatory mechanism of LOC102640519 to HOXC13. Interactions between HOXC13 and ZO-1 were measured by a luciferase reporter assay and RNA pull down assay. Our results showed that administration of VEGF significantly aggravated BBB by upregulating LOC102640519 and HOXC13 expression in vitro and vitro model of cerebral isch...Continue Reading

Citations

Apr 16, 2020·Cell Communication and Signaling : CCS·Yunxiang ZhouJianmin Zhang
Jan 9, 2019·Frontiers in Cellular Neuroscience·Ting GuoDeqin Geng
Dec 12, 2019·Frontiers in Pharmacology·Simona Federica SpampinatoMariagrazia Grilli
Feb 1, 2019·International Journal of Molecular Sciences·Shotaro Michinaga, Yutaka Koyama
Jun 21, 2021·Neuroscience Letters·Thomas A KimShaoyu Ge
Sep 10, 2021·Cellular and Molecular Life Sciences : CMLS·Qiu-Luo LiuZong-Guang Zhou

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