Vascular endothelial growth factor and semaphorin induce neuropilin-1 endocytosis via separate pathways.

Circulation Research
Anna SalikhovaArie Horowitz

Abstract

The neuropilin (Nrp)1 receptor is essential for both nervous and vascular system development. Nrp1 is unusually versatile, because it transmits both chemoattractive and repulsive signals in response to vascular endothelial growth factor (VEGF)-A and class 3 semaphorins, respectively. Both Nrp1 and VEGF receptor 2 undergo ligand-dependent endocytosis. We sought to establish the endocytic pathway of Nrp1 and to determine whether uptake is required for its signaling. Whereas Nrp1 underwent clathrin-dependent endocytosis in response to VEGFA(165) treatment, semaphorin 3C (sema3C) induced lipid raft-dependent endocytosis. The myosin VI PDZ (postsynaptic density 95, Disk large, Zona occludens-1) adaptor protein synectin was essential for Nrp1 trafficking. Sema3C failed to inhibit migration of synectin(-/-) endothelial cells, mirroring the lower migratory response of these cells to VEGFA(165). These results show that the endocytic pathway of Nrp1 is determined by its ligand and that the trafficking of Nrp1 is essential for its signaling.

References

Apr 23, 1992·Nature·S A KuznetsovD G Weiss
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·L De VriesM G Farquhar
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roben FragosoYong-Jiu Jin
Jan 17, 2003·Molecular Biology of the Cell·Lyne LabrecqueRichard Béliveau
Nov 18, 2003·Traffic·Robert G Parton, Ayanthi A Richards
Apr 7, 2004·Neuron·Carmine GuirlandJames Q Zheng
May 4, 2004·Molecular and Cellular Neurosciences·Valérie CastellaniGeneviève Rougon
Dec 15, 2004·The Journal of Cell Biology·Shingo SuzukiMasami Kojima
Feb 5, 2005·Circulation Research·Satoshi IkedaR Wayne Alexander
Jul 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Resham BhattacharyaDebabrata Mukhopadhyay
Feb 10, 2006·Molecular Biology of the Cell·Miaoliang Liu, Arie Horowitz
Mar 1, 2006·The Journal of Pharmacology and Experimental Therapeutics·Wei XuLee-Yuan Liu-Chen
Jun 3, 2006·Developmental Cell·Thomas W ChittendenMichael Simons
Jun 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ling WangXiaolei Xu
Aug 9, 2006·The Journal of Cell Biology·Maria Grazia LampugnaniElisabetta Dejana
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Samia N NaccacheArie Horowitz
Dec 13, 2006·The EMBO Journal·Krittalak ChakrabandhuAnne-Odile Hueber
Jun 19, 2007·The Journal of Biological Chemistry·Qi PanRyan J Watts
Nov 30, 2007·Nature·Winfried RömerLudger Johannes

❮ Previous
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Citations

Nov 3, 2012·Biochemistry·Matthew W ParkerCraig W Vander Kooi
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Ralf H Adams, Anne Eichmann
Jun 4, 2010·Science Translational Medicine·Olivier Feron
Nov 2, 2013·The Journal of Clinical Investigation·Lei NieMehran M Sadeghi
Jul 16, 2010·Blood·Karlien HermansPeter Carmeliet
Jul 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Puneet DangAndrew J W Furley
May 28, 2014·The Journal of Experimental Medicine·Claudio RaimondiChristiana Ruhrberg
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Mariana Melani, Brant M Weinstein
Aug 5, 2014·Microvascular Research·Matthew T MigliozziDiane R Bielenberg
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Sophie ChauvetFanny Mann
Sep 12, 2013·FEBS Letters·Himabindu Reddy SeerapuArie Horowitz
May 24, 2012·Cellular Signalling·Arie Horowitz, Himabindu Reddy Seerapu
Oct 6, 2009·Trends in Molecular Medicine·Inmaculada SeguraPeter Carmeliet
Feb 14, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Alice PleinChristiana Ruhrberg
Jun 9, 2015·The Journal of Clinical Investigation·Alice PleinChristiana Ruhrberg
Jun 30, 2011·The Biochemical Journal·Sina KochLena Claesson-Welsh
Nov 18, 2014·Biochemical Society Transactions·Anastasia Lampropoulou, Christiana Ruhrberg
Sep 19, 2009·Biochimica Et Biophysica Acta·Felix S GrünewaldKurt Ballmer-Hofer
Dec 23, 2009·Cell Adhesion & Migration·Quenten Schwarz, Christiana Ruhrberg
Oct 8, 2011·Seminars in Cell & Developmental Biology·Jennifer M James, Yoh-suke Mukouyama
May 4, 2013·Developmental Cell·Anthony LanahanMichael Simons
May 4, 2010·Developmental Cell·Anthony A LanahanMichael Simons
Jan 2, 2015·Cellular and Molecular Life Sciences : CMLS·Anna LaurenzanaFrancesca Margheri
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mingxuan ZhaoJing Wang
Jun 13, 2015·PLoS Computational Biology·Lindsay Wendel Clegg, Feilim Mac Gabhann
May 1, 2010·Neuropeptides·Alma SanchezPaula Grammas
Mar 23, 2017·Scientific Reports·Dimitris BasagiannisSavvas Christoforidis
Oct 4, 2014·Nature Communications·Hong-Bo PangErkki Ruoslahti
Mar 16, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy

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