PMID: 9418725Jan 7, 1998Paper

Vascular endothelial growth factor and vascular permeability changes in human diabetic retinopathy

Investigative Ophthalmology & Visual Science
M K MathewsG A Lutty

Abstract

The authors used histochemical analysis to determine whether increased vascular endothelial growth factor (VEGF) immunoreactivity in diabetic retinal vessels is related to increased vascular permeability, as indicated by human serum albumin (HSA) immunostaining, or to presumed retinal hypoxia as demonstrated by decreased vascularity. A correlation between VEGF and HSA in cryosections with angiopathic changes in the adenosine diphosphatase (ADPase) flat-embedded fellow retinas was sought. Because VEGF is a heparin-binding protein, the relation between VEGF and heparan sulfate proteoglycan (HSPG) immunoreactivities was also investigated. Cryopreserved eyes from 18 diabetic and 9 nondiabetic subjects removed after death were sectioned and immunohistochemical analysis was performed with antibodies against VEGF, HSA, HSPG, vWf (von Willebrand factor), and collagen IV. The fellow retinas were prepared by our ADPase flat-embedding technique to determine the degree of diabetic retinopathy. The number of positive vessels for each antibody and antibody localizations were determined by light microscopy. The average number of VEGF-stained vessels in diabetic retinas was significantly higher than in nondiabetic retinas (P = 0.04). In diabet...Continue Reading

Citations

Jun 25, 1999·The British Journal of Ophthalmology·J CaoG A Lutty
Nov 25, 2000·The British Journal of Ophthalmology·R Malhotra, K Gregory-Evans
May 17, 2008·The Journal of Clinical Investigation·Lea ScheppkeMartin Friedlander
Aug 7, 2010·Indian Journal of Ophthalmology·Kocabora M SelimOzsutcu Mustafa
Jun 8, 2012·Cold Spring Harbor Perspectives in Medicine·Motasem Al-LatayfehLloyd Paul Aiello
Mar 31, 2015·Experimental Eye Research·Ingeborg KlaassenReinier O Schlingemann
Mar 5, 2002·Ophthalmology·Stephen R FransenUNKNOWN Inoveon Health Research Group
Jun 2, 2005·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Erica L FletcherJennifer L Wilkinson-Berka
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Arthur Stevens
Jul 22, 2008·Irish Journal of Medical Science·E DervanD O'Shea
Jan 11, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Per AlbertssonKlas Norrby
Aug 2, 2013·International Journal of Molecular Sciences·Nikos G GavalasAristotle Bamias
Feb 1, 2019·Translational Vision Science & Technology·Wenqiang LiuJennifer J Kang-Mieler
Feb 24, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giulio RomeoAndrius Kazlauskas
Feb 6, 2013·Clinical Science·Jennifer L Wilkinson-BerkaAlex Agrotis

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