Vascular endothelial growth factor-expressing neural stem cell for the treatment of neuropathic pain

Neuroreport
Hye-Lan LeeYoon Ha

Abstract

Previously, we determined that vascular endothelial growth factor (VEGF) improves the survival of neural stem cells (NSCs) transplanted into an ischemic environment and effectively enhances angiogenesis. Here, we applied NSCs expressing VEGF (SV-VEGF-NSCs) to treat neuropathic pain. In this study, our goal was to verify the therapeutic effect of SV-VEGF-NSCs by transplanting the cells in a sciatic nerve injury model. We compared the amount of VEGF secreted from DsRed-NSCs (control) or SV-VEGF-NSCs and observed that SV-VEGF-NSCs have a much higher expression level of VEGF. We next investigated whether transplantation with SV-VEGF-NSCs aids functional recovery and pain reduction. We confirmed that transplantation with SV-VEGF-NSCs enhances functional recovery, pain reduction, and remyelination as well as the number of blood vessels compared with the control groups. Our results show that VEGF aids functional recovery and pain reduction in a sciatic nerve injury model.

References

Jan 1, 1989·Plastic and Reconstructive Surgery·J R BainD A Hunter
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K L JinD A Greenberg
Apr 2, 2004·Journal of Neuropathology and Experimental Neurology·Beth Brianna Murinson, John Wesley Griffin
Oct 19, 2004·Neuropathology and Applied Neurobiology·A BrockingtonP J Shaw
Aug 14, 2009·BMJ : British Medical Journal·R Freynhagen, M I Bennett
Jan 7, 2011·Neuropathology and Applied Neurobiology·F R Pereira LopesA M B Martinez
Jan 4, 2013·Journal of Neuroinflammation·Naoki KatoMasato Ogata

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Citations

Jan 31, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hye-Lan LeeYoon Ha
Jan 13, 2018·Neural Regeneration Research·Chong WangYu Wang
Feb 15, 2020·Current Stem Cell Research & Therapy·Samira AsgharzadeFatemeh Forouzanfar
Jan 19, 2021·Stem Cells International·Meichen LiuJinlan Jiang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
transmission electron microscopy
Fluorescence

Software Mentioned

SPSS

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