Vascular endothelial growth factor mRNA increases in alveolar epithelial cells during recovery from oxygen injury

American Journal of Respiratory Cell and Molecular Biology
W M ManiscalcoM H Campbell

Abstract

Destruction of pulmonary endothelial cells is characteristic of hyperoxic lung injury. During recovery from hyperoxia, pulmonary endothelial cells proliferate to regenerate the vascular endothelium. Vascular endothelial growth factor (VEGF) is a peptide growth factor that is mitogenic specifically for endothelial cells. We hypothesized that VEGF messenger RNA (mRNA) increases during recovery from acute hyperoxic lung injury. Adult rabbits were exposed to 100% oxygen for 64 h and allowed to recover in air for 0, 1, 3, and 5 days. In situ hybridization showed increased VEGF expression in alveolar epithelial cells beginning at 1 day recovery. By 3 days recovery the message was in alveolar epithelial cells throughout the lung. Compared with alveolar epithelial cells, little or no expression was noted in large vessel endothelial cells, airway cells, or smooth muscle cells. Combined in situ hybridization for VEGF and immunostaining for macrophages and other mesenchymal cells found no VEGF message in those cell types. Isolated alveolar macrophages had no detectable VEGF message. Cells expressing VEGF mRNA were enriched in alveolar type II cell preparations from recovering lung. Double in situ hybridization for VEGF and surfactant prot...Continue Reading

Citations

Feb 13, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y S NgP A D'Amore
Sep 27, 2006·Immunogenetics·Gregory S WhiteheadDavid A Schwartz
Aug 30, 2003·American Journal of Surgery·Gayle M Gordillo, Chandan K Sen
Jun 18, 2002·Microbes and Infection·Ying-Hua Li, Kjell Tullus
Mar 8, 2008·Nature Protocols·Rosemary C JonesDan G Duda
Aug 24, 2005·Antioxidants & Redox Signaling·Viren PatelChandan K Sen
Apr 24, 2008·Clinical and Experimental Pharmacology & Physiology·Gayle M GordilloChandan K Sen
May 6, 1999·American Journal of Respiratory and Critical Care Medicine·P LassusS Andersson
Feb 3, 2007·American Journal of Respiratory and Critical Care Medicine·Bernard Thébaud, Steven H Abman
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyW M Maniscalco
Dec 31, 1998·American Journal of Respiratory Cell and Molecular Biology·M J AcarreguiJ M Snyder
Nov 4, 2000·American Journal of Respiratory Cell and Molecular Biology·C G LeeJ A Elias
Sep 6, 2005·American Journal of Respiratory Cell and Molecular Biology·Su Jin ChoMichael J Acarregui
Feb 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Trent E TippleLynette K Rogers
May 16, 2009·The International Journal of Lower Extremity Wounds·Gayle M Gordillo, Chandan K Sen
Apr 1, 1997·Pediatric Research·K A Veness-MeehanA D Stiles
Nov 6, 2013·American Journal of Respiratory Cell and Molecular Biology·Min YeeMichael A O'Reilly
Feb 3, 2016·Journal of Neonatal-perinatal Medicine·V D ElbersonH S V Kumar
Oct 9, 2002·The American Journal of Pathology·Rhonda J StaverskyMichael A O'Reilly
Jun 9, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Prafulla K ChandraBenjamin S Harrison
Apr 17, 2010·The British Journal of Dermatology·S SchremlP Babilas
Jun 20, 2006·Free Radical Biology & Medicine·Vineet Bhandari, Jack A Elias
Oct 6, 2000·Clinics in Chest Medicine·D H Ingbar
Oct 23, 2013·Ultrastructural Pathology·Rosemary C Jones, Diane E Capen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

American Journal of Physiology. Lung Cellular and Molecular Physiology
Anette M KunigSteven H Abman
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Reena GhildyalJayesh Meanger
© 2021 Meta ULC. All rights reserved