PMID: 15247832Jul 13, 2004Paper

Vascular endothelial growth factor overexpression increases vascularization by murine but not human endothelial cells in cultured skin substitutes grafted to athymic mice

The Journal of Burn Care & Rehabilitation
Dorothy M SuppSteven T Boyce

Abstract

Cultured skin substitutes (CSS) consisting of fibroblasts, keratinocytes, and biopolymers are an adjunctive treatment for large burns. Because CSS lack a vascular plexus, they vascularize more slowly than split-thickness autografts. Previously, CSS were prepared with dermal microvascular endothelial cells (ECs), which formed vascular analogs at a low frequency but did not contribute to increased vascularization after grafting. The present study addressed whether keratinocytes genetically modified to overexpress vascular endothelial growth factor (VEGF), an endothelial cell mitogen, could improve the persistence and organization of ECs in CSS. CSS were prepared with control or VEGF-modified keratinocytes, with (CSS + ECs) or without added ECs, and were grafted to full-thickness wounds in athymic mice. Elevated VEGF expression was detected in VEGF-modified CSS and CSS + ECs compared with controls, but no significant difference in EC density in vitro was observed. After grafting, VEGF-modified CSS and CSS + ECs showed enhanced vascularization, and organization of human ECs into multicellular structures in CSS + ECs was observed. However, VEGF overexpression did not significantly enhance the proliferation of human ECs, suggesting t...Continue Reading

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Citations

Aug 26, 2009·Tissue Engineering. Part a·Irene MontañoErnst Reichmann
Nov 27, 2009·Tissue Engineering. Part a·Marie-Hélène RochonLucie Germain
Nov 2, 2014·ACS Applied Materials & Interfaces·Mariana Teixeira CerqueiraAlexandra Pinto Marques
May 10, 2006·Expert Review of Medical Devices·Christopher HrabchakKimberly A Woodhouse
Dec 15, 2015·Biotechnology Journal·Mohammad M MovahedniaTong Cao
Mar 8, 2013·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Fahad Karim KidwaiTong Cao

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