Vascular endothelial-specific dimethylarginine dimethylaminohydrolase-1-deficient mice reveal that vascular endothelium plays an important role in removing asymmetric dimethylarginine.

Circulation
Xinli HuYingjie Chen

Abstract

Asymmetrical methylarginines inhibit NO synthase activity and thereby decrease NO production. Dimethylarginine dimethylaminohydrolase 1 (DDAH1) degrades asymmetrical methylarginines. We previously demonstrated that in the heart DDAH1 is predominantly expressed in vascular endothelial cells. Because an earlier study showed that mice with global DDAH1 deficiency experienced embryonic lethality, we speculated that a mouse strain with selective vascular endothelial DDAH1 deficiency (endo-DDAH1(-/-)) would largely abolish tissue DDAH1 expression in many tissues but possibly avoid embryonic lethality. By using the LoxP/Cre approach, we generated the endo-DDAH1(-/-) mice. The endo-DDAH1(-/-) mice had no apparent defect in growth or development compared with wild-type littermates. DDAH1 expression was greatly reduced in kidney, lung, brain, and liver, indicating that in these organs DDAH1 is distributed mainly in vascular endothelial cells. The endo-DDAH1(-/-) mice showed a significant increase of asymmetric dimethylarginine concentration in plasma (1.41 micromol/L in the endo-DDAH1(-/-) versus 0.69 micromol/L in the control mice), kidney, lung, and liver, which was associated with significantly increased systolic blood pressure (132 m...Continue Reading

References

Feb 1, 1987·Archives of Biochemistry and Biophysics·T OgawaK Sasaoka
Oct 29, 1987·Biochemical and Biophysical Research Communications·T OgawaK Sasaoka
May 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D J DumontM L Breitman
Sep 1, 1993·The Journal of Experimental Zoology·P A Abrahamsohn, T M Zorn
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·T M SchlaegerT N Sato
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Friedrich SchulzeRainer H Böger
Apr 30, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Jan T KielsteinMarius M Hoeper
Jul 19, 2005·American Journal of Physiology. Heart and Circulatory Physiology·YingJie ChenRobert J Bache
Aug 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patrick BulauOliver Eickelberg
Oct 25, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Hiromichi KumagaiAkira Hishida
Dec 30, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Edzard SchwedhelmRainer H Böger
Feb 3, 2007·Nature Medicine·James LeiperPatrick Vallance
Feb 2, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Karsten SydowJohn P Cooke

❮ Previous
Next ❯

Citations

Apr 2, 2011·Nature Reviews. Drug Discovery·James Leiper, Manasi Nandi
Mar 30, 2011·Nature Reviews. Nephrology·Edzard Schwedhelm, Rainer H Böger
Nov 4, 2011·Current Opinion in Nephrology and Hypertension·Chris Baylis
Jan 8, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Ping ZhangRobert J Bache
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·John P Cooke, Yohannes T Ghebremariam
Feb 28, 2013·International Journal of Molecular Sciences·Shufen ChenHans Worthmann
Apr 11, 2014·BioMed Research International·Nicole LüneburgJan K Hennigs
Jul 9, 2014·Neurotoxicology·Kimio WatanabeMasaaki Nakayama
Jun 25, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kathleen J ClaesPieter Evenepoel
Jan 16, 2016·Journal of Diabetes Research·Li Lai, Yohannes T Ghebremariam
Mar 1, 2016·Nitric Oxide : Biology and Chemistry·Xiaoyu LiuYingjie Chen
Jul 24, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoyu LiuYingjie Chen
Jun 5, 2014·The Biochemical Journal·Lucio IannoneBeata Wojciak-Stothard
Nov 26, 2014·Pflügers Archiv : European journal of physiology·Kristina Kusche-VihrogEva Brand
Oct 26, 2016·Nitric Oxide : Biology and Chemistry·Da-Bin KuangXiao-Ping Chen
Apr 13, 2010·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Maike AnderssohnRainer H Böger
Jun 15, 2013·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Ryotaro AndoSeiya Okuda
Mar 31, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Ben Caplin, James Leiper
Dec 21, 2011·Circulation·Joseph A Vita
Aug 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying WangDebabrata Mukhopadhyay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.