Vascular Endothelium-Dependent and Independent Actions of Oleanolic Acid and Its Synthetic Oleanane Derivatives as Possible Mechanisms for Hypotensive Effects

PloS One
Hlengiwe P MadlalaChantal Dessy

Abstract

Plant-derived oleanolic acid (OA) and its related synthetic derivatives (Br-OA and Me-OA) possess antihypertensive effects in experimental animals. The present study investigated possible underlying mechanisms in rat isolated single ventricular myocytes and in vascular smooth muscles superfused at 37°C. Cell shortening was assessed at 1 Hz using a video-based edge-detection system and the L-type Ca2+ current (ICaL) was measured using the whole-cell patch-clamp technique in single ventricular myocytes. Isometric tension was measured using force transducer in isolated aortic rings and in mesenteric arteries. Vascular effects were measured in endothelium-intact and denuded vessels in the presence of various enzyme or channel inhibitors. OA and its derivatives increased cell shortening in cardiomyocytes isolated from normotensive rats but had no effect in those isolated from hypertensive animals. These triterpenes also caused relaxation in aortic rings and in mesenteric arteries pre-contracted with either phenylephrine or KCl-enriched solution. The relaxation was only partially inhibited by endothelium denudation, and also partly inhibited by the cyclooxygenase (COX) inhibitor indomethacin, with no additional inhibitory effect of t...Continue Reading

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Citations

Oct 17, 2008·Artificial Intelligence in Medicine·Robert W Clowes, Anil K Seth
Nov 16, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taiwo Betty AyelesoEmmanuel Mukwevho
Jun 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francisco J Luna-VázquezMoustapha Bah

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Methods Mentioned

BETA
dissection
SMA

Software Mentioned

Clampfit
Statistica
Origin
GraphPad
Myodacq
GraphPad InStat

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