PMID: 12782009Jun 5, 2003Paper

Vascular expression of matrix metalloproteinase-13 (collagenase-3) in basal cell carcinoma

Experimental and Molecular Pathology
Yukari HattoriJames Varani

Abstract

Matrix metalloproteinase-13 (MMP-13; collagenase-3) was detected in the vasculature from 17 of 20 human basal cell carcinomas as assessed by immunohistology immediately after surgery. In contrast, MMP-1 (interstitial collagenase) was detected in the vasculature of only two of the same specimens. MMP-13 reactivity was also observed in the capillaries of normal human skin taken from the wound margin. Human dermal microvascular endothelial cells as well as human umbilical vein endothelial cells were isolated in culture and examined for MMP-13 expression. By reverse transcription-polymerase chain reaction and Southern blotting, an MMP-13 transcript was detected in unstimulated endothelial cells. The transcript was upregulated in cells treated with 50 nM phorbol myristate acetate (PMA). Western blotting demonstrated the presence of an anti-MMP-13 - immunoreactive protein in culture fluid from both cell sources. Immunoreactivity was stronger in culture fluid from cells treated with interleukin-1alpha (IL-1alpha) than in culture fluid from control cells. Tumor necrosis factor-alpha (TNF-alpha) and PMA also upregulated MMP-13 expression but these agents were not as effective as IL-1alpha. Additionally, reactivity was greater in culture...Continue Reading

References

Oct 1, 1977·The Journal of Investigative Dermatology·E A BauerA Z Eisen
Dec 1, 1994·Experimental Cell Research·C J JacksonL Schrieber
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T HiroseK A Hasty
Aug 1, 1997·The Journal of Investigative Dermatology·K AirolaU K Saarialho-Kere
Aug 1, 1997·Arthritis and Rheumatism·O LindyC López-Otín
Nov 14, 1997·The New England Journal of Medicine·G J FisherJ J Voorhees
Jan 23, 1998·The International Journal of Biochemistry & Cell Biology·C J Jackson, M Nguyen
Nov 18, 1998·Current Opinion in Cell Biology·S D Shapiro
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M BalbínC López-Otín
Apr 24, 1999·The Biochemical Journal·N J Boudreau, P L Jones
Dec 15, 2000·International Journal of Molecular Medicine·Y Herouy
Apr 20, 2001·The Journal of Cell Biology·A E AplinR L Juliano
Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·M NguyenC J Jackson
Oct 5, 2001·The American Journal of Pathology·C M SheridanI Grierson

❮ Previous
Next ❯

Citations

Sep 2, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Zornitsa I ZlatarovaElisabeth M Messmer
Nov 22, 2011·Molecular Aspects of Medicine·Diego SbardellaMassimo Coletta
Jul 11, 2009·The American Journal of Pathology·Noriko HattoriYasunori Okada
Feb 14, 2012·Vascular Pharmacology·Andrew C Newby
Apr 18, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryuji YasumatsuSule Cataltepe
Sep 2, 2020·Experimental Dermatology·Pilvi RiihiläVeli-Matti Kähäri
Nov 20, 2004·International Journal of Cancer. Journal International Du Cancer·Marc A LafleurErik W Thompson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Basal Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
M BalbínC López-Otín
Matrix Biology : Journal of the International Society for Matrix Biology
Nanjun WuJeffrey M Davidson
© 2022 Meta ULC. All rights reserved