Vascular Kinin B1 and B2 Receptors Determine Endothelial Dysfunction through Neuronal Nitric Oxide Synthase

Frontiers in Physiology
Thássio R R MesquitaSandra Lauton-Santos

Abstract

B1- and B2-kinin receptors are G protein-coupled receptors that play an important role in the vascular function. Therefore, the present study was designed to evaluate the participation of kinin receptors in the acetylcholine (ACh)-induced vascular relaxation, focusing on the protein-protein interaction involving kinin receptors with endothelial and neuronal nitric oxide synthases (eNOS and nNOS). Vascular reactivity, nitric oxide (NO·) and reactive oxygen species (ROS) generation, co-immunoprecipitation were assessed in thoracic aorta from male wild-type (WT), B1- (B1R(-/-)), B2- (B2R(-/-)) knockout mice. Some vascular reactivity experiments were also performed in a double kinin receptors knockout mice (B1B2R(-/-)). For pharmacological studies, selective B1- and B2-kinin receptors antagonists, NOS inhibitors and superoxide dismutase (SOD) mimetic were used. First, we show that B1- and B2-kinin receptors form heteromers with nNOS and eNOS in thoracic aorta. To investigate the functionality of these protein-protein interactions, we took advantage of pharmacological tools and knockout mice. Importantly, our results show that kinin receptors regulate ACh-induced relaxation via nNOS signaling in thoracic aorta with no changes in NO·...Continue Reading

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Citations

Jun 27, 2019·Malaria Journal·Priscilla D S VenturaMarcos L Gazarini
Apr 21, 2020·Journal of Translational Medicine·Stéphanie RuizVincent Minville
May 1, 2019·Life Sciences·Thássio Ricardo Ribeiro MesquitaSandra Lauton-Santos

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Methods Mentioned

BETA
electrophoresis
immunoprecipitation

Software Mentioned

GraphPad Prism
WinDaq
ImageJ

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