Vascular labeling of luminescent gold nanorods enables 3-D microscopy of mouse intestinal capillaries

ACS Nano
Shiue-Cheng TangHsing-Yu Tuan

Abstract

In this study, we aimed to use gold nanorods (Au-NRs) as luminescent substrates for labeling of the mouse intestinal blood vessels for tissue imaging. The labeled intestine was subjected to 3-D confocal microscopy to reveal the intricate morphology of the intestinal capillaries. Using the Au-NR's unique near-infrared excitation and visible fluorescence emission, we observed low noise background compared to the tissue's high autofluorescence from blue laser excitation. We took advantage of this sharp contrast in optical properties to achieve 3-D visualization of the intestinal microstructure and vasculature with capillary-level resolution. This new optical application demonstrates the Au-NR's distinctive properties in vascular labeling and fluorescence microscopy for 3-D illustration of intestinal vasculature.

References

Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Mar 30, 2005·Archives of Pathology & Laboratory Medicine·Mark Li-cheng WuBela Molnar
Aug 13, 2005·Microvascular Research·Dino J RavnicMoritz A Konerding
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haifeng WangJi-Xin Cheng
Nov 22, 2005·Nature Methods·Jeff W Lichtman, José-Angel Conchello
Nov 22, 2005·Nature Methods·Fritjof Helmchen, Winfried Denk
Jul 21, 2006·The Journal of Physical Chemistry. B·Kohei ImuraHiromi Okamoto
Jan 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Danielle K Smith, Brian A Korgel
May 7, 2008·The American Journal of Pathology·Livija DebanSilvio Danese
May 10, 2008·Journal of Biomedical Optics·Valery V TuchinAlvin T Yeh
Jul 23, 2008·Nano Letters·Jill E MillstoneChad A Mirkin
Nov 21, 2008·Advanced Materials·Ling TongJi-Xin Cheng
Dec 25, 2008·Gastroenterology·John Virostko, Alvin C Powers
Oct 31, 2009·ACS Nano·Hai-Qiao Wang, Thomas Nann
Nov 7, 2009·ACS Nano·Christopher TaborMostafa A El-Sayed
Aug 24, 2010·Gastroenterology·Ya-Yuan Fu, Shiue-Cheng Tang
Dec 28, 2009·Advanced Materials·Xiaohua HuangMostafa A El-Sayed

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Citations

May 24, 2012·Chemical Reviews·Marc VendrellYoung-Tae Chang
Jan 31, 2013·Molecular Pharmaceutics·Aneta J MieszawskaDavid P Cormode
Sep 26, 2013·Nanoscale·Joanna Olesiak-BanskaMarek Samoc
Jan 31, 2012·Chemical Society Reviews·Tennyson L Doane, Clemens Burda
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Sep 8, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y-A LiuS-C Tang
Nov 7, 2012·Chemical Society Reviews·Huanjun ChenJianfang Wang
Aug 9, 2016·Journal of Colloid and Interface Science·Jingjie LuoCorinne Petit
Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Yuan FuShiue-Cheng Tang
May 5, 2021·The Science of the Total Environment·Xiaoquan Huang, Meng Tang
Oct 12, 2017·Biochimie·Luc Pénicaud

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