Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) delays and induces escape from senescence in human dermal microvascular endothelial cells

Oncogene
Y WatanabeH F Dvorak

Abstract

Like most other normal cells, human endothelial cells possess a limited replicative life span, and, after multiple passages in vitro, develop an arrest in cell division referred to as replicative senescence. For many cell types senescence can be delayed by oncogenes or tumor suppressor genes or prevented altogether by malignant transformation; however, once developed, senescence has been regarded as irreversible. We now report that a cytokine, vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), significantly delays senescence in human dermal microvascular endothelial cells (HDMEC). Typically, VPF/VEGF-treated HDMEC could be cultured for at least 15-20 more population doublings (PD) than control cells. Protection from senescence was reversible in that subsequent withdrawal of VPF/VEGF returned cells to the senescent phenotype. Expression of several cell cycle-related genes (p21, p16 and p27) was significantly reduced in VPF/VEGF-treated cells but p53 expression was not significantly altered. Of particular importance, VPF/VEGF was able to rescue senescent HDMEC, restoring them to proliferation, to a more normal morphology, and to reduced expression of a senescence marker, neutral beta-galactosidase. Taken ...Continue Reading

Citations

Feb 27, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R G Faragher, D Kipling
Mar 28, 2008·Current Urology Reports·Rishi Sawhney, Fairooz Kabbinavar
Mar 3, 2005·The Journal of Nutritional Biochemistry·Mark P Mattson, Ruiqian Wan
Feb 28, 2002·American Journal of Ophthalmology·Michael J TolentinoGerard A Lutty
Aug 26, 1998·Progress in Cardiovascular Diseases·W B BatchelorB H Strauss
Aug 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·M V BackerJ M Backer
Apr 20, 2001·Maturitas·D Kipling
Jun 1, 2000·Experimental Gerontology·F Bringold, M Serrano
Jun 27, 2002·Journal of the American College of Cardiology·Hannes Franz AlberFranz Weidinger
Jul 3, 2003·Trends in Cardiovascular Medicine·Janice A NagyHarold F Dvorak
Feb 28, 2004·Trends in Cardiovascular Medicine·M Clauss
May 16, 2003·Vascular Pharmacology·D O Bates, S J Harper
Feb 28, 2003·Ageing Research Reviews·David R RiddleRobin J Lichtenwalner
Jan 31, 2009·Journal of Medical Imaging and Radiation Oncology·L G SahlerD L Waldman
Nov 28, 2007·Annual Review of Pathology·Janice A NagyHarold F Dvorak
Dec 22, 2011·Journal of Inflammation·Marie At MatiasCamile S Farah
Jul 18, 2002·Japanese Journal of Pharmacology·Tetsuya NakamuraMasahiko Kurabayashi
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G van der PluijmC Löwik
Jun 20, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Feb 18, 2016·Clinical and Experimental Medicine·Diego GuidolinDomenico Ribatti
Dec 13, 2005·Expert Review of Anticancer Therapy·W Kimryn RathmellBrian I Rini
Dec 22, 2007·Expert Review of Anticancer Therapy·Fiona J CollinsonGordon C Jayson
Mar 1, 2006·Expert Opinion on Pharmacotherapy·Brian I Rini
Jun 21, 2007·Endothelium : Journal of Endothelial Cell Research·Domenico Ribatti
Jan 19, 2016·Journal of Molecular and Cellular Cardiology·Basak IcliMark W Feinberg
Feb 8, 2011·Current Problems in Surgery·Jason P GlotzbachMichael T Longaker
Aug 12, 2014·Science China. Life Sciences·WenGong Wang
Jul 6, 2010·Experimental Gerontology·Louise BoisenPeter Kristensen
Sep 26, 2009·Clinics in Podiatric Medicine and Surgery·Simon E Smith, Thomas S Roukis
Jun 27, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Michael L H WongChristopher M Hovens
May 2, 2002·The Journal of Investigative Dermatology·Edwin ChangG Scott Herron
Jan 24, 2006·Clinical Genitourinary Cancer·Brian I Rini
Nov 26, 2008·The Journal of Surgical Research·Philip BaoHarold Brem
Oct 13, 2007·Critical Reviews in Oncology/hematology·John S LamRobert A Figlin

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