Vascular relaxation of canine visceral arteries after ischemia by means of supraceliac aortic cross-clamping followed by reperfusion.

Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
José G CiscatoC E Piccinato

Abstract

The supraceliac aortic cross-clamping can be an option to save patients with hipovolemic shock due to abdominal trauma. However, this maneuver is associated with ischemia/reperfusion (I/R) injury strongly related to oxidative stress and reduction of nitric oxide bioavailability. Moreover, several studies demonstrated impairment in relaxation after I/R, but the time course of I/R necessary to induce vascular dysfunction is still controversial. We investigated whether 60 minutes of ischemia followed by 30 minutes of reperfusion do not change the relaxation of visceral arteries nor the plasma and renal levels of malondialdehyde (MDA) and nitrite plus nitrate (NOx). Male mongrel dogs (n = 27) were randomly allocated in one of the three groups: sham (no clamping, n = 9), ischemia (supraceliac aortic cross-clamping for 60 minutes, n = 9), and I/R (60 minutes of ischemia followed by reperfusion for 30 minutes, n = 9). Relaxation of visceral arteries (celiac trunk, renal and superior mesenteric arteries) was studied in organ chambers. MDA and NOx concentrations were determined using a commercially available kit and an ozone-based chemiluminescence assay, respectively. Both acetylcholine and calcium ionophore caused relaxation in endoth...Continue Reading

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Citations

Oct 17, 2019·International Journal of Molecular Sciences·Ricardo O S SoaresOrlando Castro-E-Silva
Jun 26, 2020·Cell Death & Disease·Weifeng YaoZiqing Hei

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Methods Mentioned

BETA
blood drawn
MDA
Assay

Software Mentioned

GraphPad
Prism

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