Vascular smooth muscle cell stiffness and adhesion to collagen I modified by vasoactive agonists

PloS One
Zhongkui HongGerald A Meininger

Abstract

In vascular smooth muscle cells (VSMCs) integrin-mediated adhesion to extracellular matrix (ECM) proteins play important roles in sustaining vascular tone and resistance. The main goal of this study was to determine whether VSMCs adhesion to type I collagen (COL-I) was altered in parallel with the changes in the VSMCs contractile state induced by vasoconstrictors and vasodilators. VSMCs were isolated from rat cremaster skeletal muscle arterioles and maintained in primary culture without passage. Cell adhesion and cell E-modulus were assessed using atomic force microscopy (AFM) by repetitive nano-indentation of the AFM probe on the cell surface at 0.1 Hz sampling frequency and 3200 nm Z-piezo travelling distance (approach and retraction). AFM probes were tipped with a 5 μm diameter microbead functionalized with COL-I (1 mg\ml). Results showed that the vasoconstrictor angiotensin II (ANG-II; 10-6) significantly increased (p<0.05) VSMC E-modulus and adhesion probability to COL-I by approximately 35% and 33%, respectively. In contrast, the vasodilator adenosine (ADO; 10-4) significantly decreased (p<0.05) VSMC E-modulus and adhesion probability by approximately -33% and -17%, respectively. Similarly, the NO donor (PANOate, 10-6 M),...Continue Reading

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Citations

Dec 5, 2015·Frontiers in Physiology·Nancy L SehgelGerald A Meininger
Mar 2, 2017·Cellular and Molecular Life Sciences : CMLS·Alexandra C FinneyA Wayne Orr
Dec 6, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Yi ZhuYong Zhou
Mar 8, 2018·Cardiovascular Research·Patrick LacolleyAlberto P Avolio
Jan 9, 2019·Frontiers in Physiology·Huang HuangGerald A Meininger
Aug 31, 2020·Bi︠u︡lletenʹ Sibirskoĭ Medit︠s︡iny·A A SulginV Y Sidorov
Jul 19, 2020·International Journal of Molecular Sciences·Stephanie CicaleseSatoru Eguchi
May 2, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Patricia E McCallinhartAaron J Trask
May 15, 2020·Journal of Applied Physiology·Timothy M MahanesAnalia S Loria
Jul 13, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hicham Labazi, Aaron J Trask

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Methods Mentioned

BETA
AFM

Software Mentioned

Matlab

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