Vascular smooth muscle contraction evoked by cell volume modulation: role of the cytoskeleton network

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Svetlana V KoltsovaSergei N Orlov

Abstract

Previously, we reported that hyposmotic swelling evoked transient vascular smooth muscle cell (SMC) contraction that was completely abolished by L-type Ca(2+) channel blockers. In contrast, sustained contraction revealed in hyper- and isoosmotically-shrunken SMCs was insensitive to L-type channel blockers and was diminished in Ca(2+)-free medium by only 30-50%. Several research groups reported cell volume-dependent cytoskeleton network rearrangements. This study examines the role of cytoskeleton proteins in cell volume-dependent contraction of endothelium-denuded vascular smooth muscle rings (VSMR) from the rat thoracic aorta. Hyperosmotic shrinkage and hyposmotic swelling were triggered by modulation of medium osmolality; isosmotic shrinkage was induced by VSMR transfer from hypo- to isosmotic medium. The relative content of globular (G) and fibrillar (F) actin was estimated by fluorescence microscopy. Hyperosmotic shrinkage and hyposmotic swelling led to elevation of the F-actin/G-actin ratio by 2.5- and 1.8-fold respectively. Contraction of shrunken and swollen VSMR was insensitive to modulators of microtubules such as vinblastine, colchicine and docetaxel. Microfilament disassembly by cytochalasin B resulted in dramatic att...Continue Reading

References

Dec 1, 1991·The American Journal of Physiology·K R HallowsP A Knauf
Apr 1, 1994·European Journal of Pharmacology·K Obara, H Yabu
Oct 1, 1995·The Journal of Membrane Biology·I LevitanS S Garber
May 1, 1996·The Journal of Surgical Research·B C SheridanD A Fullerton
Apr 1, 1996·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·S Y SaitoH Karaki
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M P WalshK G Morgan
May 1, 1996·The American Journal of Physiology·S N OrlovP Hamet
Apr 16, 1998·Canadian Journal of Physiology and Pharmacology·A S Battistella-PattersonG L Wright
Sep 30, 1999·Experimental Cell Research·S F PedersenE K Hoffmann
Oct 19, 1999·Microscopy Research and Technique·J H Henson
Jul 27, 2000·American Journal of Physiology. Cell Physiology·M NakamuraN Sperelakis
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R J PaulM S Kolodney
Dec 23, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·E A PapakonstantiC Stournaras
Aug 25, 2001·Journal of Muscle Research and Cell Motility·D ZhangD R Swartz
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marilyn J CipollaGeorge Osol
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Gui-Rong LiClive M Baumgarten
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S F PedersenJ W Mills
Sep 11, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·José Pedro L Nunes
Nov 28, 2002·Autonomic & Autacoid Pharmacology·J A C Filipe, J P L Nunes
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ge-Xin WangIlia A Yamboliev
Aug 5, 2004·Pflügers Archiv : European journal of physiology·Yana J AnfinogenovaSergei N Orlov
Nov 5, 2004·American Journal of Physiology. Cell Physiology·Shunsuke YanoKoichi Nakayama
Jan 15, 2005·Journal of Vascular Research·S Wijetunge, A D Hughes
Jun 1, 2006·Acta Physiologica·C Di Ciano-OliveiraA Kapus
Feb 20, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Sergei N Orlov
Aug 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Sergei N Orlov, Alexander A Mongin
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva

❮ Previous
Next ❯

Citations

Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·George J C YeKevin Kit Parker
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Cai TangFlorian Lang
Jan 12, 2010·Current Opinion in Nephrology and Hypertension·Sergei N OrlovPavel Hamet
May 13, 2020·Journal of Cell Science·Priyanka S RanaMichael A Model

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.