Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated

The Plant Cell
B. Keller, C Baumgartner

Abstract

In French bean, the glycine-rich cell wall protein GRP 1.8 is specifically synthesized in the vascular tissue. To identify cis-acting sequences required for cell type-specific synthesis of GRP 1.8, expression patterns of fusion gene constructs were analyzed in transgenic tobacco. In these constructs, the uidA (beta-glucuronidase) gene was placed under control of 5' upstream deletions as well as internal deletions of the GRP 1.8 promoter. Four different cis-acting regulatory regions, SE1 and SE2 (stem elements), a negative regulatory element, and a root-specific element, were found to control the tissue-specific expression. Deletion of the negative regulatory element resulted in expression of the uidA gene in cell types other than vascular cells. The SE1 region was essential for expression in several cell types in the absence of further upstream regulatory sequences. Full-length promoters having insertions between the negative regulatory element and SE1 strongly expressed the gene in nonvascular cell types in stems and leaves. Thus, vascular-specific expression of the GRP 1.8 promoter is controlled by a complex set of positive and negative interactions between cis-acting regulatory regions. The disturbance of these interactions ...Continue Reading

References

May 1, 1990·The Plant Cell·D E de OliveiraJ Botterman
Nov 26, 1984·Nucleic Acids Research·M Bevan
Feb 3, 1984·Science·R B HorschN Hoffmann
Apr 14, 1989·Science·P N Benfey, N H Chua

❮ Previous
Next ❯

Citations

Jan 1, 1995·Plant Molecular Biology·C A Loopstra, R R Sederoff
May 31, 2006·Journal of Plant Research·Shinobu Satoh
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Hiroo Fukuda
May 6, 2003·Annual Review of Phytopathology·Howard J AtkinsonMichael J McPherson
Jan 9, 2009·BMC Plant Biology·Alexandre EvrardThomas Eulgem
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shunhong DaiRoger N Beachy
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Yuanya LiPing Wu
May 13, 2009·Journal of Biotechnology·Xiaomeng LvHai Lu
Nov 2, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lance A DavidsonDouglas W DeSimone
Mar 26, 2011·The Plant Journal : for Cell and Molecular Biology·Roberto Ruiz-MedranoWilliam J Lucas
Oct 19, 2004·Biochimica Et Biophysica Acta·Patricia GuzzardiElisabeth Jamet
Aug 1, 1992·Current Opinion in Genetics & Development·C Niehrs, E M De Robertis
Dec 17, 2009·Plant Signaling & Behavior·Amanda MangeonGilberto Sachetto-Martins
Aug 2, 2001·Plant & Cell Physiology·L B SmartA B Bennett
Jan 1, 2014·Horticulture Research·Manjul DuttJude W Grosser
Oct 1, 1993·The New Phytologist·Matilde Josè, Pere Puigdomènech
Feb 1, 1995·The New Phytologist·A M BoudetJ Grima-Pettenati
Jun 20, 2000·Biochimica Et Biophysica Acta·G Sachetto-MartinsD E de Oliveira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.